Simultaneous determination of ten plant growth regulators and fungicides in bean sprouts using Qu ECh ERS- UPLC- MS / MS
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摘要: 建立了一种同时分析豆芽中10种植物生长调节剂和杀菌剂的分散固相萃取-超高效液相色谱-串联质谱法（UPLC-MS/MS）的检测方法。样品经过1%酸化乙腈提取,PSA、C18、GCB吸附剂净化,UPLC-MS/MS多反应监测模式测定,外标法定量,检测结果显示:多菌灵、6-苄基氨基嘌呤、吲哚乙酸、赤霉素、恩诺沙星、2,4-二氯苯氧乙酸、多效唑、咪鲜胺、涕灭威和甲基托布津10种药物浓度在5.0200.0μg/L范围内呈良好线性关系,相关系数均大于0.99,加标回收率为80.4%99.8%,相对标准偏差（RSD%）为2.0%7.8%,方法检出限为0.22.6μg/kg。该方法简单、快速、可靠。串联质谱法在很大程度上减少了基质干扰,减少了假阳性现象的产生。
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关键词:
- 植物生长调节剂 /
- 杀菌剂 /
- 超高效液相色谱-串联质谱法 /
- 分散固相萃取 /
- 豆芽
Abstract: A method was established based on Qu ECh ERS- ultra- high performance liquid chromatography- triple quadrupole mass spectrometry( UPLC- MS / MS) to simultaneously determine 10 kinds of plant growth regulators and fungicides residues in bean sprouts. The sample were extracted with 1% acidic acetonitrile,then dehydrated and cleaned using PSA,C18 and GCB.The Qq Q mass spectrometer was operated in a multiplereaction monitoring mode and qualified using external standard method. The results showed that All of residues( Carbendazim,6- Benzylaminopurine,Indole- 3- acetic acid,Gibberellin,Enrofloxacin,2,4- Dichlorophenoxyacetic acid,Paclobutrazol,Prochloraz,Aldicarb,Thiophanate methyl) were in a good linear relationship between 5.0 and200.0 μg / L and the relative coefficient were more than 0.99,the recoveries were in the range of 80.4% ~99.8%,the relative standard deviations were 2.0% ~ 7.8%,the limits of detection of the method were varied from 0.2 to2.6 μg / kg. The method is simple,rapid and reliable. It can be suitable for detection and confirmation of these ten plant growth regulators and fungicides in bean sprouts products.-
Keywords:
- plant growth regulator /
- fungicide /
- UPLC-MS / MS /
- Qu ECh ERS /
- bean sprouts
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