Isolation and identification of two bacteria from rotten Myrica rubra
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摘要: 以腐烂杨梅为研究对象,采用平板划线分离与斜面纯化技术,筛选出两株编号为YMS-7和YMN-5的细菌,分别提取两株菌的DNA后,扩增16S r DNA序列并克隆测序,获得的基因序列在Ez Bio Cloud数据库中进行同源性比对,构建16S r DNA系统发育树,结合生理生化分析,将YMS-7和YMN-5分别鉴定为类芽孢杆菌Paenibacillus agaridevorans与嗜气芽孢杆菌Bacillus aerophilus。
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关键词:
- 杨梅 /
- 菌株鉴定 /
- 16S r DNA基因 /
- 系统发育分析
Abstract: In this study,two strains of bacteria coded as YMS-7 and YMN-5 were isolated from rotten Myrica rubra using plate streaking and slant culture methods.After extraction of DNA,16 S r DNA from both strains were amplified for sequencing and homology analysis using Ez Bio Cloud database.Two phylogenetic trees were established based on these sequences of 16 S r DNA,combined with physiological and biochemical characteristics. Consequently,YMS-7 and YMN-5 were identified as Paenibacillus agaridevorans and Bacillus aerophilus,respectively.-
Keywords:
- Myrica rubra /
- identification /
- 16S r DNA /
- phylogenetic analysis
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