Protective effect of Zige lyophilized powder on brain microvascular endothelial cells after hypoxia- reoxygenation injury
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摘要: 探讨梓葛冻干粉针对脑微血管内皮细胞（BMECs）缺氧/复氧损伤的保护作用。取新生10 d的SD大鼠乳鼠,分离、培养脑皮层微血管原代内皮细胞,并利用DMEM无糖培养基、三气培养箱缺氧容器（缺糖缺氧2 h）及5%CO2培养箱复氧培养24 h复制缺氧/复氧模型,MTT法BMECs细胞活力,Western blot法检测MMP-9的蛋白表达。结果显示,与模型组相比,梓葛冻干粉针(49.00、98.00μg·m L-1)能促进缺氧/复氧模型中BMECs增殖;梓葛冻干粉针(24.50、49.00、98.00μg·m L-1)能显著抑制BMECs细胞中MMP-9蛋白表达升高（p<0.05）;提示梓葛冻干粉针对脑微血管内皮细胞损伤的保护作用可能与降低MMP-9蛋白表达有关。Abstract: To investigate the protective effect of Zige lyophilized powder on brain microvascular endothelial cells( BMECs) after hypoxia- reoxygenation injury. BMECs of the 10 d rats were isolated and cultured,the model of BMECs with the hypoxia- reoxygenation injury was estabished by DMEM sugar- free medium,three gas incubator oxygen container( lacking oxygen and sugar for 2 h),and 5% CO2 incubator for 24 h after oxygen training. The proliferation of endothelial cells was detected by MTT method. The protein expression levels of MMP- 9 were detected by WB method. Zige lyophilized powder( 49.00,98.00 μg·m L-1) could promote BMECs proliferation in hypoxia- reoxygenation model.The increasing level of MMP-9 protein in a hypoxia- reoxygenation injury on cultured BMECs was inhibited significantly by Zige lyophilized powder( 24.50,49.00 and 98.00 μg·m L-1)( p < 0.05). It showed that the possible mechanism of Zige lyophilized powder for injection on protecting the cell integrity would be associated with the decreasing MMP-9 protein expression.
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Keywords:
- Zige lyophilized powder for injection /
- BMECs /
- MMP-9 /
- Hypoxia-reoxygenation injury
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