Analysis of volatile compounds in Cordyceps cicadae and submergedly cultured mycelia of Isaria cicadae
-
摘要: 为研究不同提取方法对野生蝉花与发酵菌丝体挥发性化合物种类及含量的影响,首先通过ITS基因序列分析、菌落及产孢结构的形态特征对菌株GZU1205241进行鉴定。然后通过顶空固相微萃取（HS-SPME）和水蒸气蒸馏对野生蝉花和发酵菌丝体的挥发性化合物进行提取,并利用气相色谱-质谱（GC-MS）对两者挥发性化合物进行了测定。结果表明,菌株GZU1205241为蝉棒束孢菌。野生蝉花和发酵菌丝体分别含有56和58种挥发性物质,其中25种为共有物质。野生蝉花主要以酸类（90.08%）物质为主,其中油酸的相对含量达到了45.09%,棕榈酸的含量为21.18%,亚油酸的含量为9.44%。发酵菌丝体中主要也以酸类物质（86.57%）物质为主,其中棕榈酸的相对含量达到了38.44%,亚油酸的相对含量达到了18.58%,油酸的相对含量为12.83%。两种方法获得的野生蝉花及发酵菌丝体的挥发性成分种类及相对含量上有明显差别,水蒸气蒸馏法鉴定出的挥发性成分较多,而顶空固相微萃取鉴定出的挥发性成分比较少。但不论是蒸气蒸馏法还是顶空固相微萃取,野生蝉花及发酵菌丝体的挥发性成分都是以脂肪酸为主。Abstract: In order to determine the volatile compounds in Cordyceps cicadae and submergedly cultured mycelia of Isaria cicadae,the strain GZU1205241 was identified by the sequence analysis of a r DNAITS fragment and the traditional identification methods(morphological characteristics of the colony and sporulation structure).Then the volatile compounds of wild Cordyceps cicadae mycelium and fermentation volatile compounds were extracted by headspace solid phase microextraction(HS-SPME) and steam distillation and determined by gas chromatography and mass spectrometry. The results showed that the strain GZU1205241 was Isaria cicadae.Cordyceps cicadae and mycelia of Isaria cicadae,respectively,contained 56 and 58 volatile compounds.Hereinto,25 compounds in both wild and mycelia. The main volatile compounds of Cordyceps cicadae mainly were acids,and the relative content of oleic acid,hexadecanoic acid and linoleic acid was 45.09%,21.18% and9.44%,respectively. The main volatile compounds of fermented mycelia were acids,and the relative content of palmitic acid,linoleic acid and oleic acid was 38.44%,18.58% and 12.83%,respectively. The volatile composition and relative content of wild Cordyceps Cicadae and fermented mycelia obtained by steam distillation and headspace solid phase micro extraction had obvious differences. The volatile composition were more by water vapor distillation. Acid were the main volatile compounds of Cordyceps cicadae and fermented mycelia obtained by steam distillation and headspace solid phase micro extraction.
-
Keywords:
- Cordyceps cicadae /
- volatile compounds /
- HS-SPME /
- GC-MS /
- Isaria cicadae
-
[1] 杨介钻,卓佳,陈柏坤,等.蝉拟青霉多糖对老年大鼠免疫功能的调节作用[J].中国中药杂志,2008,33(3):292-295. [2] 谢炜,郭月芳,盛雨辰.蝉花菌丝体对慢性肾功能衰竭大鼠的治疗作用[J].中国医药工业杂志,2011,42(10):770-772. [3] 刘玉宁,陈以平,王立红,等.蝉花菌丝抗大鼠肾小管间质纤维化的实验研究[J].中国中西医结合肾病杂志,2011,12(3):243-245. [4] 徐红娟,莫志宏,余佳文,等.蝉花抗真菌活性成分的分离纯化研究[J].天然产物研究与开发,2010,22(5):794-797,835. [5] 翁梁.野生蝉花与人工蝉花多糖的提取及抑菌实验[J].农业科技与装备,2011(11):22-24. [6] 闫梅霞,杨元超,侯微,等.药用菌多糖的抗肿瘤作用研究[J].安徽农业科学,2011,39(18):10799-10801. [7] 蔡菊芬,姜志明,卢红阳,等.蝉拟青霉不同纯化组分对体外抗肿瘤作用的基础研究[J].中华中医药学刊,2010,28(4):760-764. [8] 陈安徽,樊美珍,邵颖,等.蝉拟青霉代谢产物抑制单胺氧化酶和抗肿瘤活性研究[J].食品科学,2009,30(11):216-218. [9] 陈安徽,李春如,樊美珍.蝉拟青霉代谢产物清除DPPH自由基和抗真菌活性的研究[J].菌物学报,2008.27(3):405-412. [10] 欧翔,谭艾娟,吕世明.蝉拟青霉酯酶的产酶条件优化[J].贵州农业科学,2013,41(8):144-147. [11] 雷帮星,康冀川,何劲,等.四种虫草菌丝体中腺苷和虫草菌素含量的研究[C].中国菌物学会第五届会员代表大会暨2011年学术年会,2011. [12] 陈安徽,陈宏伟,徐洋,等.蝉花虫草中核苷类成分的分离纯化和鉴定[J].食品科学,2013,34(1):131-134. [13] Lee YS,KM Choi,MH Choi,et al.Serine palmitoyltransferase inhibitor myriocin induces growth inhibition of B16F10 melanoma cells through G(2)/M phase arrest[J].Cell Prolif,2011,44(4):320-9.
[14] Tamura K,D Peterson,N Peterson,et al.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Molecular biology and evolution,2011,28(10):2731-2739.
计量
- 文章访问数: 194
- HTML全文浏览量: 19
- PDF下载量: 220
下载:
