Change of pork flavor on endogenous lipase by electronic nose
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摘要: 为考察内源性脂肪酶对肉品风味的影响,本研究通过单因素和正交实验优化建立最佳作用条件,并结合感官评定和电子鼻检测酶处理前后肉品风味的变化。结果表明:当作用时间为40 min,加酶量为30%,作用温度为37℃时,感官评定分析肉品风味评分达到最高值;酶解后挥发性风味物质含量在硫化物、有机硫化物、芳香类及含乙醇类等成分含量变化明显;主成分分析法(PCA)和线性判别式分析法(LDA)进一步说明添加内源性脂肪酶在一定程度上有效地改善了肉品的香气。Abstract: In order to study the effect of endogenous lipase on the pork flavor,the optimum reaction condition was studied through single factor and orthogonal experiment. The electronic nose was used to detect the changes of pork flavor by different enzyme treatment.The results showed that when the time was 40 min,the amount of enzyme was 30%,temperature was 37 ℃,the pork flavor score reached the highest point by sensory analysis.The composition of sulfides,organic sulfides,aromatic and ethanol was increased significantly,meanwhile,Principal Component Analysis( PCA) and Linear Discriminant Analysis,LDA( LDA) analysis further illustrated that the flavor of pork was effectively improved after adding endogenous lipase in a certain extent.
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Keywords:
- endogenous lipase /
- electronic nose /
- flavor /
- pork
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[1] 刘海洲,吴小飞,牛佰慧,等.脂肪酶在食品工业中的应用与研究展[J].粮食加工,2008,33(5):55-57. [2] 刘树立,王春艳,王华.酶制剂在肉制品加工中的应用进展[J].肉类研究,2007,(1):35-37. [3] Foraker A B,Chandra M K,Peter W S.Current perspectives on the cellular uptake and trafficking of riboflavin[J].Advanced Drug Delivery Reviews,2003,55:1467-1483.
[4] 范芳.双水相萃取技术的应用进展[J].化学与生物工程,2011,28(7):16-20. [5] Hasan Farina,Shah Aamer Ali,Hameed Abdul.Industrial applications of microbial lipases[J].Enzyme and MicrobialTechnology,2006,39(2):235-251.
[6] 刘俊花,葛玉,张宝善.食品风味酶的研究进展[J].中国酿造,2005,2:6-8. [7] 刘虹蕾,缪铭,江波,等.微生物脂肪酶的研究与应用[J].食品工业科技,2012,33(12):376-380. [8] 黄业传,李凤,黄甜,等.利用电子鼻和气质联用研究腊肉挥发性风味物质的形成规律[J].食品工业科技,2014,35(6):73-77. [9] 崔方超,李婷婷,杨兵,等.电子鼻结合GC-MS对草鱼脱腥前后风味变化的研究[J].食品科学,2014(20):150-154. [10] 徐永霞,刘滢,姜程程,等.电子鼻结合气质联用法分析大菱鲆冷藏过程中挥发性成分变化[J].分析与检测,2014,40(1):193-197. [11] 黄业传,贺稚非,李洪军,等.皮下脂肪和肌内脂肪对猪肉风味的作用[J].中国农业科学,2011,44(10):2118-2130. [12] 莫树平,林嘉慧,柏建玲,等.肉制品中的风味物质及其分析方法[J].中国调味品,2007,(1):25-30. [13] Gupta R,Gupta N,Rathi P.Bacterial lipases:an overview of Production,Purification and biochemical Properties[J].Applied Microbiology and Biotechnology,2004,64:763-781.
[14] Chmidt DC,Sztajer H,Stoeckl ein W,et al.Screening,purification and properties of a thermophilic lipase from Bacillus thermocatenulatus[J].Biochim Biopsy’s Acta,1994,1214(1):43-53.
[15] 郑俏然.真空包装调理猪肉制品的开发研究[J].食品科技,2012,37(2):142-147. [16] Fernandez M.Efect of the Addition of Pancreatic Lipase on the Ripening of Dry Fermented Sausages.Part II.Free fatty acids,short-chain fatty acids,carbonyls and sensory quality[J].Meat Science,1995,40:351-362.
[17] Diaz O,Fernandez M,Garcia de Femando G D,et al.Effect of the addition of pronase E on the proteolysis in dry fermented sausages[J].Meat Science,1993,34(2):205-216.
[18] Zapelena M J,Zalacain I,Paz-de-Pena M,et al.Effect of the addition of a neutral proteinase from Bacillus subtilis on nitrogen fractions and texture of Spanish fermented sausage[J].J of Agricultural and Food Chemistry,1997,45(7):2798-2801.
[19] Fernandez M,de la Hoz L,Diaz O,et al.Effect of the addition of pancreatic lipase on the ripening of dry-fermented sausages-Part 2.Free fatty acids,short-chain fatty acids,carbonyls and sensory quality[J].Meat Science,1995,40(3):351-362.
[20] 封莉,邓绍林,黄明,等.脂肪酶(Palatase)对中式香肠脂肪降解、氧化和风味的影响[J].食品科学,2015,36(1):51-57. [21] Eva Hierro,Lorenzo de la Hoz,Juan A.Ordonez.Contribution of Microbial and Meat Endogenous Enzymes to the Lipolysis of Dry Fermented Sausages[J].Agric Food Chem.,1997,(45):2989-2995.
[22] Fidel Toldra,Monica Flores,M Concepcion Aristoy.Pattern of Muscle Proteolytic and Lipolytic Enzymes from Light and Heavy Pigs[J].Sci Food Agric.,1996,(71):124-128.
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