Extraction of flavonoids from valerian optimized by response surface method
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摘要: 本文以产自贵州地区的缬草为实验材料,以乙醇为浸提试剂,探究缬草中黄酮类化合物的提取工艺。方法:在乙醇浓度、料液比、浸提温度和浸提时间四个单因素实验基础上,采用响应面设计方法优化提取工艺参数。结果表明:最佳提取工艺为乙醇浓度53.0%,料液比1∶29 g/m L,浸提温度73℃,浸提时间2.6 h。在此条件下,实际得到的黄酮类化合物得率为2.133%,与理论提取率相比较,其RSD为3.5%。结论:响应面法优化的缬草中黄酮类化合物提取工艺稳定,具有可靠性。Abstract: The extraction process of flavonoid compounds originated from Valeriana ofecnilis( produced from Guizhou province) was investigated using ethanol as the extraction solvent. Method: Four factors,including concentration of Ethanol,ratio of raw material to solvent,extracting temperature and time,were optimized by response surface method.The optimum conditions were as follows: the concentration of ethanol was 53.0%; ratio of material to solvent was 1 ∶ 29 g / m L; extraction temperature was 73 ℃ and extraction time was 26 h. Under the optimum conditions,the difference of between real value of 2.133% and theoretic value in rate of extraction was about 3.5%. Conclusion: Response surface method was a reliable tool to optimize the extraction of flavonoid compounds from Valeriana ofecnilis.
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Keywords:
- Valeriana ofecnilis /
- Flavonoids /
- Response surface method /
- Yield
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