Optimization of fermentation medium of Lactobacillus plantarum KLDS1.0386 for bile salt hydrolase production
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摘要: 为了提高植物乳杆菌Lactobacillus plantarum KLDS1.0386的胆盐水解酶产量,本实验通过响应面法对其发酵培养基进行优化。通过单因素实验、Plackett-Burman实验、最陡爬坡实验和Box-Behnken实验,最终获得最优培养基为:葡萄糖18.2 g/L、蛋白胨15.03 g/L、酵母粉9.97 g/L、乙酸钠3.13 g/L、柠檬酸氢二铵2.00 g/L、磷酸氢二钾2.00 g/L、硫酸锰0.25 g/L、硫酸镁0.58 g/L。优化前植物乳杆菌KLDS1.0386产胆盐水解酶的比酶活为1.04 U/mg,经过培养基的优化后,胆盐水解酶的比酶活为3.37 U/mg,比优化前提高了3.24倍。且实验结果与模型预测结果误差在允许范围内,说明该模型可以投入使用。Abstract: In order to improve the bile salt hydrolase production of Lactobacillus plantarum KLDS1.0386,the response surface method was used to optimize the medium composition. The optimal values of the culture medium were as follows(g/L) :glucose 18.2 g/L,peptone 15.03 g/L,yeast extract 9.97 g/L,sodium acetate3.13 g/L,diammonium hydrogen citrate 2.00 g/L,K_2HPO_4 2.00 g/L,Mn SO_40.25 g/L,Mg SO_4 0.58 g/L. Before optimizing,the activity of bile salt hydrolase was 1.04 U/mg,Under the optimal conditions,specific enzyme activity of bile salt hydrolase was 3.37 U/mg,increasing 3.24 times than before. The experimental result was consistent with the value predicted by the mathematical model. which showed the model can be put into use.
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