Purification and antioxidant activity of polysaccharide from Boletus aereus in Yunnan
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摘要: 通过对铜色牛肝菌多糖进行分离纯化和活性分析,为野生铜色牛肝菌的进一步推广和应用提供理论依据。本研究采用热水浸提法提取的粗多糖为原料,并利用DEAE-52纤维素柱层析和Sephadex G-100柱层析对所获得的粗多糖进行纯化,得到两种单一多糖PB-1A、PB-2A,比旋光度和紫外扫描方法鉴定多糖的纯度。通过体外抗氧化实验评价体系研究,比较铜色牛肝菌粗多糖及纯化后的多糖PB-1A、PB-2A对羟基自由基、DPPH自由基的清除活性以及总还原能力。结果表明:铜色牛肝菌多纯化糖前后的对羟基自由基、DPPH自由基的清除活性以及总还原能力呈现一定效量关系,且均为PB-2A>PB-1A>铜色牛肝菌粗多糖。Abstract: The purification and activity analysis of Boletus aereus polysaccharides can provide a theoretical basis for the further application and popularization of B. aereus. In the present work,crude polysaccharides of Bronze Boletus was extracted by hot water and then successively purified with DEAE-52 ion exchange column and Sephadex G-100 gel permeadun column. Two polysaccharides,named PB-1A and PB-2A,were obtained after purification. The purity of obtained polysaccharide was determined by specific rotation method and UV spectral scanning. The total reducing power,hydroxyl free radical and DPPH free radical scavenging activity of crude polysaccharides,PB-1A and PB-2A were determined by in vitro antioxidant evaluation system. The results demonstrated that the total reducing power,hydroxyl free radical and DPPH free radical scavenging activity of B. aereus polysaccharides before and after purification showed a dose- dependent manner, with activities being PB-2A>PB-1A>crude polysaccharides of B. aereus.
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