Separation of β- sesquiphellandrene from ginger oleoresin
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摘要: 应用超临界二氧化碳萃取姜油树脂,选用乙醇-水作流动相,HPD-100大孔树脂作固定相分离出其中的烯类物质,并用高效液相色谱法进行跟踪检测确定含烯类的洗脱段。对比不同展开剂下烯类物质在硝酸银硅胶板上的层析效果,最终选用V正己烷∶V乙酸乙酯=95∶5的溶剂作流动相分离其中的β-倍半水芹烯。GC/MS检测结果表明,分别选用HPD-100大孔树脂和硝酸银硅胶作为固定相可以将姜油树脂中的β-倍半水芹烯很好的分离出来,其峰面积百分数达88.73%,收率为84.05%。通过进一步HPLC检测证明β-倍半水芹烯具有紫外吸收,其特征吸收波长为232 nm。Abstract: A ethanol- water solution was used as mobile phase,and the HPD- 100 macroporous resin as the stationary phase to separate the vinyl part from ginger oleoresin extracted by supercritical carbon dioxide,and HPLC was used for detecting vinyls tracing.Different developing solvents of vinyls on argentated silica gel G precoated plate were compared,Vhexane∶ V(ethylacetate)= 95 ∶ 5 was determined as the mobile phase to sepatateβ- sesquiphellandrene. The GC / MS test results indicated that HPD- 100 macroporous resin and silver nitrate modified silica gel were separately used as the stationary phase which could effectively separateβ- sesquiphellandrene from ginger oleoresin with 88.73% area percentage and 84.05% recovery. Through further HPLC analysis,β- sesquiphellandrene was found its existence of UV absorption at 232 nm.
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Keywords:
- silver nitrate modified silica gel /
- β-sesquiphellandrene /
- separation
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[1] Pinder A R.萜类化学[M].北京:科学出版社,1964,135-143. [2] 倪明红,陈钟瑛,严秉淳.光学活性姜烯类倍半萜烯的合成及其抗生育活性的探讨[J].华东化工学院学报,1988,14(6):677-678. [3] 贾雁高.生姜精油中姜烯成分的分离纯化[D].济南:山东大学,2010. [4] Vries B DE.Quantitative separation of highel fatty acid methyl esters by adsorption chromatography on silica impregnated with silver nitrate[J].JAOCS.1963,40(5):184-186.
[5] Bennett B,Larter S R.Quantitative Separation of Aliphatic and Aromatic Hydrocarbons Using Silver Ion-Silica Solid-Phase Extraction[J].J Anal Chem.2000,72(5):1039-1044.
[6] 欧阳长庚,卢文彪.利用硝酸银处理的硅胶色谱方法分离和提纯中药有效成分的思考[J].广州中医药大学学报,2004,21(4):319-321. [7] 黄汉昌,朱宏吉,姜招峰.银离子配位色谱法分离香茅次油中的β-榄香烯的研究[J].无机化学学报,2009,25(3):433-438. [8] 王艳,杜爱玲,薛岩明,等.D4020大孔树脂分离姜油树脂中的姜酚[J].山东大学学报,2011,41(3):148-153. [9] Ghosh A,Hoque M Dutta.Separation of polyunsaturated fatty acid esters by argentation column chromatography on silicic acid[J].J Chromatography,1972,69(1):207-208.
[10] 张汆,阚建全,陈宗道.硝酸银硅胶纯化α-亚麻酸的研究[J].离子交换与吸附,2005,21(1):47-54. [11] Snyder L R,Dolan J W,Grant J R.Gradient elution in highperformance liquid chromatography:I.Theoretical basis for reversed-phase systems[J].Chromatography,1979,165(1):3-30.
[12] Teris A,Van B,Gerrit P.Isolation and Identification of the Five Major Sesquiterpene Hydrocarbons of Ginger[J].J Phytochemical Analysis,1991,2(1):26-34.
[13] 王艳,杜爱玲,杜爱琴.银离子配位柱层析法分离姜油树脂中的姜烯[J].精细化工,2012,29(7):673-677.
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