Influence of ethyl acetate extract of Centella asiatica on abilities of learning and memory and the expressions of APP and BACE1 in SAMP8
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摘要: 目的:探讨积雪草乙酸乙酯提取物对快速老化模型小鼠SAMP8记忆行为和大脑淀粉样肽前体蛋白（APP）、淀粉蛋白前β位分解酶1（BACE1）基因表达的影响。方法:将SAMP8随机分为模型对照组、积雪草乙酸乙酯提取物高、中、低剂量组（以生药量计为40、20、10 g·kg-1）和石杉碱甲组（0.386 mg·kg-1）,8只/组,各药物组按设计剂量连续给药2个月,模型对照组给予等体积双蒸水灌服。采用Morris水迷宫（MWM）方法观察SAMP8行为学改变,应用半定量反转录聚合酶链反应（RT-PCR）测定SAMP8脑内APP和BACE1 m RNA含量的变化。结果:Morris水迷宫测试中,与模型对照组比较,积雪草乙酸乙酯提取物高、中剂量组和石杉碱甲组的逃避潜伏期显著缩短（p<0.01）、停留时间显著延长（p<0.01）及平台象限百分比显著增大（p<0.01或p<0.05）,积雪草乙酸乙酯提取物中剂量组和石杉碱甲组的寻求次数比模型对照组明显增多（p<0.01）;RT-PCR结果显示积雪草乙酸乙酯提取物高、中剂量组和石杉碱甲组SAMP8大脑的APP和BACE1 m RNA表达水平显著降低（p<0.05）。结论:积雪草乙酸乙酯提取物对SAMP8学习记忆功能有明显改善作用,并能降低脑内APP和BACE1 m RNA的表达。
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关键词:
- 积雪草乙酸乙酯提取物 /
- SAMP8 /
- 记忆行为 /
- 大脑淀粉样肽前体蛋白 /
- 淀粉蛋白前β位分解酶1
Abstract: Objective:To investigate Ethyl acetate extract of Centella asiatica on the abilities of learning and memory and contents of APP,BACE1 m RNA in SAMP8 with Alzheimer'disease. Methods:SAMP8 were randomly divided into 5 groups:the model control group,Ethyl acetate extract of Centella asiatica high-dose(40 g·kg-1),middose(20 g·kg-1),low-dose(10 g·kg-1) group and huperzin A group(0.386 m g·kg-1). Each mouse was administered intragastricly for 2 months. The same amount of double distilled water was given to the model control group. The test of Morris water maze(MWM) was used to examine the changes of the abilities of learning and memory. The contents of APP and BACE1 m RNA in brain tissue were assayed by reverse transcription polymerase chain reaction(RT-PCR). Results:Morris water maze demonstrated that mice treated with Ethyl acetate extract of Centella asiatica and huperzine A,mean escape latencies were significantly decreased( p < 0. 01), the residence times were significantly prolonged( p < 0. 01), the percentages of total swimming distance in the platform quadrant were significantly increased(p<0.01,p<0.05) and seek times in mid-dose group of the ethyl acetate extract of Centella asiatica and huperzine A group were more than that of the model control group(p<0.01). The result of RT-PCR showed that high,mid-dose groups of the ethylacetate extract of Centella asiatica and huperzine A group could reduce the contents of APP and BACE1 m RNA. Conclusion:Ethyl acetate extract of Centella asiatica had a significant relieving effect on the lesion of the abilities of learning and memory,it could reduce the contents of APP and BACE1 m RNA in SAMP8.-
Keywords:
- ethyl acetate extract of Centella asiatica /
- SAMP8 /
- learning and memory /
- APP /
- BACE1
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