Bioconcentration characteristics of Cadmium( Ⅱ) by Boletus edulis
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摘要: 本文研究了美味牛肝菌液体发酵对不同浓度镉（Ⅱ）(Cd2+,0、16、32、48、64、80 mg/L)的生物富集特性。结果表明,Cd2+处理显著抑制了美味牛肝菌菌丝体的生长（p<0.05）,生长抑制率为50%的Cd2+浓度为56 mg/L。菌丝体对Cd2+的生物富集量可高达3335.7 mg/kg DW,富集系数为52.1（p<0.05）。菌丝体中可溶性总糖和多糖含量随Cd2+浓度的升高呈现先上升后下降的趋势,Cd2+处理菌丝体中的多糖含量均高于无Cd2+组（p<0.05）;菌丝体中可溶性蛋白含量随Cd2+浓度的升高先降低后上升,80 mg/L Cd2+处理组显著高于无Cd2+组（p<0.05）。Cd2+处理对菌体过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性呈现显著的"低促高抑"的影响趋势（p<0.05）,而过氧化物酶（POD）活性被显著抑制（p<0.05）。表明美味牛肝菌对Cd2+具有很强的生物富集能力,可能是菌体的特定成分如多糖、蛋白等协同抗氧化酶系增强了菌体对Cd2+的耐受性和富集性能。Abstract: The bioconcentration characteristics of cadmium( Ⅱ) with different concentrations( Cd2+,0,16,32,48,64 and 80 mg / L) by Boletus edulis under liquid fermentation were investigated.The results showed that the treatment with increasing Cd2+concentrations significantly inhibited mycelia growth( p < 0.05),and 50% inhibitory concentration was 56 mg / L.Mycelia showed significant accumulation capacity for Cd2+,with the highest content of3335.7 mg Cd per kg mycelia( DW),and the bioconcentration factor was 52.1( p < 0.05). The contents of total soluble sugars and polysaccharides in mycelia were increased firstly and then decreased with increasing Cd2+concentrations.The content of polysaccharides in mycelia treated with Cd2+significantly higher than that of control( p < 0.05). Soluble protein contents were decreased firstly and then increased with increasing Cd2+concentrations,with the peak at 80 mg / L Cd2+( p < 0.05). The activities of CAT and SOD were significantly increased by lower Cd2+concentrations but inhibited by high Cd2+concentrations( p < 0.05).The activities of POD were significantly inhibited by Cd2+treatment( p < 0.05).
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Keywords:
- Boletus edulis /
- cadmium /
- bioconcentration /
- food safety
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