Renal protection of Oleuropein on diabetic rats and relevant mechanism
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摘要: 为了探讨橄榄苦苷对糖尿病大鼠肾组织的保护作用及其机制。将雄性SD大鼠随机分成正常组、糖尿病模型组、橄榄苦苷组和阳性药物贝那普利组,采用腹腔注射链脲佐菌素（STZ）65 mg/kg建立糖尿病大鼠模型1周后,给予橄榄苦苷或阳性药物贝那普利治疗7周。检测大鼠尿、血液和肾脏的各项指标。结果显示,与模型对照组相比,橄榄苦苷明显降低糖尿病大鼠肾脏指数、血糖、血脂、Scr、BUN、UA、UALB、UAER水平,降低MDA含量（p<0.05,p<0.01）,提高血清Ig G、Ig M和补体C3、C4含量、肾脏端粒酶、SOD、CAT活性（p<0.05,p<0.01）,抑制肾脏TGF-β1和Smad4的表达（p<0.05,p<0.01）。提示橄榄苦苷对STZ诱导的糖尿病大鼠肾脏具有明显的保护作用,其机制可能与其提高了肾脏的抗氧化能力和端粒酶活性及机体的免疫功能,抑制肾脏TGF-β1和Smad4的表达有关。Abstract: To investigate the protective mechanism of oleuropein on the kidney of diabetic rats, male SD rats were divided into normal control group, diabetic model group, oleuropein treated group and positive benazepril control group based on their body weights. The diabetic animal model was made by a single intraperitoneal injection of65 mg / kg streptozotocin ( STZ) .One week after successful modeling, the diabetic rats were treated with oleuropein or positive benazepril for seven weeks, and then indexes of their urine, blood and kidneys were measured.Results:Compared with diabetic model group, the oleuropein treated rats showed significant decrease in levels of renal index, blood glucose, blood lipid, serum Scr, BUN, UA, UALB, UAER and MDA, increase in levels of serum Ig G, Ig M and complement C3, C4, telomerase, SOD and CAT, and reduce in content of MDA. Meanwhile, the use of oleuropein resulted in decrease of expression of TGF- β1 and Smad4 in diabetic rats. These results indicated that oleuropein had a protective effect on the STE- induced renal tissue injury in diabetic rats, due to enhancing renal antioxidant capacity, telomerase activity and immune function, as well as inhibiting expression of TGF- β1 and Smad4.
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Keywords:
- oleuropein /
- diabetic nephropathy /
- antioxidant enzyme /
- TGF-β1 /
- Smad4
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