Optimization of ultrasonic- assisted enzymatic extraction of polysaccharides from Cantharellus cibarius by Plackett- Burman design and response surface methodology
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摘要: 为优化超声波协同酶法提取鸡油菌子实体中多糖的工艺条件,在单因素实验的基础上,首先采用PlackettBurman实验设计筛选出影响鸡油菌多糖得率的四个主要因素:酶解p H、固液比、超声功率和酶解时间。随后以鸡油菌多糖得率为响应值,采用Box-Behnken实验设计及响应面分析法优化提取条件,得到最佳提取工艺参数为:酶解p H5.5、固液比1∶46（g/m L）、超声功率210 W、酶解时间68 min。在此条件下多糖理论得率为12.08%、实际得率为11.91%、相对误差1.41%,表明Plackett-Burman设计结合响应面分析法可以很好地对鸡油菌多糖超声协同酶法提取工艺进行优化,可为鸡油菌多糖的广泛应用提供一定的技术支撑。
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关键词:
- 鸡油菌多糖 /
- Plackett-Burman设计 /
- 超声波 /
- 纤维素酶
Abstract: To optimize the ultrasinic- assisted enzymatic extraction of polysaccharides from Cantharellus cibarius, on the base of the single factor experiment results, enzymatic hydrolysis p H, solid- liquid ratio, ultrasonic power and enzymatic hydrolysis time were identified as main factors that influence polysaccharides yield with the Plackett-Burman experiment design firstly.Then the Box- Behnken experiment design and response surface analysis were adopted to optimize the extraction conditions with polysaccharides yield as the response value. The optimized technological parameters were as follows: p H5.5, solid- liquid ratio 1 ∶ 46 ( g / m L) , ultrasonic power 210 W and enzymatic hydrolysis time 68 min. Under these conditions, the theoretical and actual crude polysaccharides yield were 12.08% and 11.91%, respectively, with a relative error of 1.41%, which indicated that the combination of Plackett- Burman design and response surface analysis can well optimize the extraction technology of polysaccharides from Cantharellus cibarius with the ultrasinic- assisted enzymatic extraction method and it can provide certain technical support for the widely used of Cantharellus cibarius. -
[1] 卫亚丽, 王茂盛, 连宾.鸡油菌研究进展[J].食用菌, 2006, 28 (2) :1-3. [2] 黄年来.中国食用菌百科[M].北京:中国农业出版社, 1997. [3] 罗成, 周达, 鲁晓翔, 等.鸡油菌多糖的提取及其抗氧化研究[J].食品研究与开发, 2010, 31 (7) :155-159. [4] Mdachi SJM, Nkunya MHH, Nyigo VA, et al.Amino acid composition of some Tanzanian wild mushrooms[J].Food Chemistry, 2004, 86 (2) :179-182.
[5] 罗成, 鲁晓翔, 周达.鸡油菌多糖降血糖作用研究[J].食品工业科技, 2010, 31 (12) :333-334. [6] 史碧波.微波辅助提取鸡油菌多糖的工艺研究[J].安徽农业科学, 2011, 39 (30) :18533-18535. [7] 史碧波, 王学雪, 罗晓妙.超声波辅助提取鸡油菌多糖的研究[J].食品与机械, 2012, 28 (2) :152-155. [8] 史碧波, 罗晓妙, 刘满.超声波在鸡枞菌多糖提取中的应用研究[J].现代食品科技, 2008, 24 (1) :64-66. [9] 魏凤玉, 康家胜, 张宇.纤维素酶法提取黄芪多糖的动力学[J].过程工程学报, 2012, 12 (4) :839-843. [10] 李顺峰, 刘兴华.真姬菇子实体多糖的提取工艺优化[J].农业工程学报, 2008, 24 (2) :281-284. [11] Zhou JY, Yu XJ, Ding C, et al.Optimization of phenol degradation by Candida tropicalis Z-04 using Plackett-Burman design and response surface methodology[J].Journal of Environmental Sciences, 2011, 23 (1) :22-30.
[12] 潘春梅, 王辉, 任敏.响应面法优化L-谷氨酰胺发酵培养基的研究[J].生物技术通讯, 2013, 19 (4) :528-531. [13] 李赓, 徐涛, 王平.响应面设计法优化超声波辅助提取金针菇多糖的研究[J].安徽农学通报, 2010, 16 (23) :23-26. [14] 刘航, 国旭丹, 马雨洁, 等.超声辅助提取苦荞麦多糖工艺优化及其体外抗氧化研究[J].食品科学, 2013, 34 (14) :45-50. [15] 程伟, 秦俊哲, 杜军国, 等.桑黄多糖超声波协同纤维素酶法提取的工艺研究[J].现代食品科技, 2012, 28 (6) :662-666. [16] 靳文娟, 鲁晓翔.超声波法提取鸡油菌多糖及其抗氧化性研究[J].安徽农业科学, 2011, 39 (27) :16545-16547.
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