Study on the proteolytic ability and ACE inhibitory activity of Lactobacillus delbrueckii QS306 to milk
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摘要: 通过研究Lactobacillus delbrueckii QS306在10%的还原脱脂牛乳中的生长特性,探讨培养基中添加核酸关联物质时对蛋白质水解能力及其水解产物对血管紧张素转化酶（ACE）抑制活性的影响。结果表明,菌株QS306在10%的还原脱脂牛乳中生长缓慢,产酸量和生物量逐渐上升,在37℃培养第4 d时,滴定酸度和生物量分别达到68.24（°T）和6.91（OD410 nm×10）,蛋白质水解能力为623.21μg·m L-1Tyr。在培养基中添加核酸关联物质鸟嘌呤（Guanine）时蛋白质水解能力提高了43.7%,ACE抑制活性可达到61.99%。Abstract: The growth characteristics of Lactobacillus delbrueckii QS306 in reconstituted skim milk( 10% wt / wt) was observed,and its proteolytic ability and angiotensin- converting enzyme( ACE) inhibitory activity were investigated by the addition of growth promoting substances.The results showed that Lactobacillus delbrueckii QS306 was slow- growing in skim milk,and its acid production and biomass were increased gradually. The titratable acidity,biomass and protein decomposition at 37 ℃ for 4 days were 68.24( ° T),6.91( OD410 nm× 10) and 623.21 μg·m L- 1Tyr,respectively.The proteolytic ability of strain QS306 was increased by 43.7% and the( ACE) inhibitory activity was61.99% when adding Guanine in skim milk.
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Keywords:
- Lactobacillus /
- proteolytic ability /
- ACE inhibitory activity
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[1] 杨洁彬,郭兴华,傅晓丽,等.乳酸菌-生物学基础及应用[M].北京:中国轻工业出版社.1996:1-83. [2] 李平兰.乳酸菌细菌素研究进展[J].微生物学通报,1998,5:295-299. [3] Maruyama S,Suzuki H,A peptide inhibitor of angiotensin I converting enzyme in the tryptichydrolysate of casein[J].Agricultural Biological Chemistry,1982,46(5):1393-1394.
[4] Nakamura Y,Yamamoto N,Sakai K,et al.Purification and Characterization of Angiotensin I—Converting Enzyme Ihibitors from Sour Milk[J].J Dairy Science,1995,78:777-783.
[5] Johnston J I,Franz V L.Renin-angiotensin system:a dual tissue and hormonal system for cardiovascular control[J].JHypertens,1992,10(S):13-26.
[6] 王朝晖,程龙献,王祥,等.卡托普利注射液对高血压急症的降压作用[J].临床心血管病杂志,1998,14(4):215-216. [7] Hans M.Overview on milk protein-derived peptides[J].Internat Dairy,1998,8(5):363-373.
[8] Yamamoto N,Akino A,Takano T.Antihypertensive effects of different kinds of fermented milk in spontaneously hypertensive rats[J].Biosci Biotech Biochem,1994,58(4):776-778.
[9] Mgobbetti,Ferranti P,Smacchi E.Production of angiotensin-I-converting-enzyme-inhibitory peptides in fermented milks started by Lactobacillus delbrueckii subsp.bulgaricus SS1and Lactococcus lactissubsp.Cremoris FT4[J].Appl Environm Microbio,l 2000,66(9):3898-3904.
[10] 吕加平,董晓波,肖锐.发酵乳品中乳酸菌生物量测定方法的研究[J].肉品卫生,1997,(2):3-7. [11] Minervini F,Algaron F,Rizzello CG,et al.Angiotensin IConverting Enzyme Inhibitory and Antibacterial Peptides form Lactobacillus helveticus PR4 Proteins Hydrolyzed Caseins of Milk form Six Species[J].American Society for Microbiology,2003,69:5297-5305.
[12] 中島昭正.脱脂乳へのアミノ酸添加が乳酸菌の酸生成に及ぼす效果日本家政學會誌47(11):2007,1085-1091. [13] Hernández-Ledesma B,Miralles B,Amigo L,et al.Identification of antioxidant and ACE-inhibitory peptides in fermented milk[J].J Food Agric Sci,2005,85:1041-1048.
[14] Michaelidou A,Alichanidis E.Isolation and Identafication of some major water-soluble peptides in feta cheese[J].J Dairy Sci,2011,81:3109-3116.
[15] 中島昭正.脱脂乳への核酸關連物質の添加が乳酸菌の酸生成に及ぼす效果.日本家政學會誌48(1):2008,11-18. [16] 商靓靓,陈卫,田丰伟,等.具有血管紧张素转换酶抑制活性的乳酸菌筛选及特性研究[J].中国乳品工业,2006,34(4):24-28.
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