Physicochemical properties of fish oil nanoliposomes made by thin-film rehydration-ultrasonic and ethanol injection-DHPM
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摘要: 通过比较优化后的薄膜-探头式超声法（脂质体Ⅰ）和注乙醇-DHPM法（脂质体Ⅱ）制备的鱼油脂质体的理化性质及稳定性,初步评价两种方法的优点与缺点。分别测定脂质体Ⅰ和脂质体Ⅱ的包封率、载量、粒径分布、Zeta电位以及形态观察和4、25℃条件下贮藏p H变化、贮藏粒径变化。结果显示脂质体Ⅱ相比脂质体Ⅰ的平均粒径、多分散指数（PDI）、Zeta电位小,TEM、AFM显示脂质体Ⅱ比脂质体Ⅰ的颗粒完整性更好。4℃和25℃条件下分别贮存35d的对比结果表明脂质体Ⅱ相比于脂质体Ⅰ在储存过程中的p H、平均粒径变化小,贮藏稳定性更好。Abstract: The merits and disadvantages of thin-film rehydration-ultrasonic method and ethanol injection-DHPM were evaluated by comparing the physicochemical properties of fish oil(FO) nano liposomes prepared under individual optimum conditions.(liposome I and liposome II,respectively) The polydisperse index,particle size,zeta potential,transmission electron microscopy and p H of liposomes was detected. Result:Nanoliposomes Ⅱexhibited small eraverage particle,PDI and zeta potentialsize,than nano liposomes I. TEM and AFM analysis revealed that the particle integrity of liposome II was better than liposomes I. Conclusion :Compared with nanoliposomes I,nanoliposomes Ⅱ exhibites greater characteristies,and ethanol injection-DHPM could be a potent technique for the preparation of nanoliposomes.
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