Study on extraction and antibacterial activity of the red pigment from Crataegus jingxiensis Li peel
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摘要: 研究了微波辅助提取靖西大果山楂皮中红色素的最佳工艺条件,考察乙醇浓度、料液比、微波功率、微波时间对色素提取的影响,通过正交实验对上述条件进行了优化,并采用滤纸片琼脂扩散法测定了红色素的抑菌活性。结果表明:山楂皮红色素提取的最佳条件为:乙醇浓度60%,料液比1∶50(g/m L),微波功率500W,微波时间60s,提取2次。抑菌实验结果显示,红色素对金黄色葡萄球菌、表皮葡萄球菌、大肠杆菌、伤寒杆菌均有一定的抑制作用。Abstract: Studied the best condition for the extraction of the red pigment from the Crataegus jingxiensis Li peel through microwave-assisted technology,and investigated the influence of alcohol concentration,liquid ratio,microwave power,microwave minute on the extraction of the pigment. In the whole process of the experiment,the above conditions were optimized by the orthogonal experiment. Antibacterial activities of red pigment were determined by paper disk diffusion. Results showed that the best conditions for the extraction of the red pigment from the Crataegus jingxiensis Li peel were as follows:alcohol concentration 60%,liquid ratio 1∶50(g/m L),microwave power 500 W,microwave minute 60 s and extraction twice. The results of the antibacterial experiments showed that the red pigment had certain inhibitory effect on Staphylococcus aureus, Staphylococcus epidermidis,Escherichia coli and Salmonella enterica.
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Keywords:
- Crataegus jingxiensis Li /
- peel /
- extraction /
- pigment /
- antibacterial activities
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