高产阿魏酸酯酶菌株的筛选及其产酶条件优化

基金项目:

湖南省科技计划项目(2012NK3072)；

详细信息

Selection of high-yield ferulic acid esterase strain and optimization of enzyme production conditions

摘要: 通过初筛、复筛从茯砖茶中筛选到了1株产阿魏酸酯酶活力较高的菌株,经个体形态和菌落特征初步鉴定其为冠突散囊菌;以阿魏酸酯酶酶活为衡量指标,选取黑毛茶含水量、接种量、培养温度、培养时间为因子,研究确定筛选出来的冠突散囊菌进行固态发酵黑毛茶的产酶条件。结果表明:黑毛茶固态发酵实验中,黑毛茶含水量26%,接种量1‰,温度30℃条件下培养4d,其酶活力最高可以达到142m U/g。
- 阿魏酸酯酶 /
- 酶活 /
- 发酵型茶 /
- 冠突散囊菌 /
- 筛选
Abstract: Through preliminary and secondary screening,a strains with high enzyme activity which could produce feruloyl esterase was screened out from Fu-brick tea,according to its individual and colony characteristics,it was identified as Eurotium cristatum. Feruloyl esterase enzyme activity was used as measurable indicator,water content,inoculums-size,temperature and time was chosen as variables,the solid-state fermentation conditions of chosen Eurotium cristatum were optimized. The results showed that black primarytea the optimal condition was 1‰ inoculums size,26% moisture content and cultivated at 30℃ for 4 days,the highest enzyme activity reached 142 m U/g in the black Maocha solid-state fermentation experiment.
- ferulic acid esterase /
- activity /
- fermented tea /
- Eurotium cristatum /
- screening