Effects on culture conditions for β-carotene contents of Dunaliella sp.
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摘要: 为了优化杜氏藻的培养条件,对采自山西运城盐湖的杜氏藻（Dunaliella sp.）采用单因素和正交实验方法,研究了温度、盐度和氮、磷、铁元素对其细胞增殖和β-胡萝卜素积累的影响。结果显示杜氏藻的β-胡萝卜素含量与吸光度OD453nm之间存在良好的线性关系,利用吸光值表征杜氏藻β-胡萝卜素含量步骤简单,数据准确,适于高效快速对样品进行测定。杜氏藻在2025℃,1.02.0mol/L Na Cl时,较有利于β-胡萝卜素积累。杜氏藻生长至对数期后,高浓度的氮源、磷源都不利于β-胡萝卜素的积累,而铁源在实验期间,对杜氏藻β-胡萝卜素的积累无影响。进一步优化,培养基中有利于β-胡萝卜素积累的Na NO3、KH2PO4和Fe C6H5O7的浓度最优组合为0.6200、0.0256、0.0010g/L。本实验得到了较适合杜氏藻积累β-胡萝卜素的培养基,为今后当地开发利用高产β-胡萝卜素藻株奠定了理论基础。Abstract: The single factor and orthogonal experiments were performed to optimize the cultivating conditions of Dunaliella sp. which was collected from salt lake in Yuncheng, Shanxi, China. Temperature, Na Cl, N, P and Fe were studied for effects on β-carotene contents of Dunaliella sp. The results showed that there was a good linear relationship between β-carotene contents of Dunaliella sp. and OD453 nm. The β-carotene contents of Dunaliella sp. was measured by the method of optical density which was simple, accurate and efficient.Dunaliella sp. was cultured under 20~25℃ and 1.0~2.0mol/L (Na Cl) that were conducive to the β-carotene accumulation. When Dunaliella sp. cells grow to the logarithmic phase, high concentrations of N and P were not conducive to the accumulation of β-carotene, and the concentration of Fe had no effect on the accumulation ofβ-carotene. The optimum concentrations of Na NO3, KH2PO4 and Fe C6H5O7 for β-carotene accumulation were0.6200, 0.0256, 0.0010g/L. The medium for β-carotene accumulation of Dunaliella sp. was optimized for lay the theoretical foundation of future development and utilization of local high β-carotene production algae strains.
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Keywords:
- Dunaliella sp. /
- cell density /
- β-carotene /
- optimization of culture condition
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