Optimization of polyphenol extraction from Sedum Aizoon L.by response surface methodology
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摘要: 目的:利用响应面法对费菜中多酚物质的提取条件进行优化。方法:单因素实验基础上选取实验因素与水平,根据Box-Behnken Design（BBD）实验设计原理,采用三因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因素,以费菜多酚物质含量为响应值作响应面分析实验。结果:在分析各个因素的显著性和交互作用后,得出费菜多酚物质浸提的最佳工艺条件为乙醇体积分数67%,浸提温度41℃,浸提时间2h,料液比1∶25（g/m L）,以焦性没食子酸为标准品,费菜多酚物质一次提取含量可达60.90mg/g。结论:响应曲面回归方程与实验结果拟合性好,此模型合理可靠,可用于实际预测。Abstract: Objective:The main research object was Sedum Aizoon L. Besides, an optimization of extraction process for from Sedum aizoon L. was established. Methods:Based on the single factor analysis, a 3-factor, 3-level Box-Behnken experimental design was applied to evaluate the effect of the three factors, including ethanol concentration, ratio of material to liquid and extraction temperature. Results:The optimal extraction conditions were ethanol concentration of 67% (v/v) , extraction temperature 41℃, extraction time 2h, solid-to-liquid ratio 1∶25 (w/v) . Under such extraction conditions, the experimental yield of polyphenols obtained after the first extraction was up to 60.90mg/g, which was close to the model-predicted value. Conclusion :The established regression equation for polyphenols yield had an excellent goodness of fit. Therefore it was credible and could be used for practical prediction.
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Keywords:
- Sedum Aizoon Linn /
- polyphenols /
- response surface methodology
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