Study on acid precipitation technology of protein in cassava starch wastewater and its influencing factors
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摘要: 以木薯黄浆废水为研究对象,分析木薯黄浆废水主要组成成分,并对其中的蛋白质组分进行有效地沉淀。通过实验测定木薯黄浆废水蛋白质的等电点在3.5~4.5之间。通过单因素实验条件,选择pH、沉淀时间、温度和黄浆废水蛋白质浓度为变量,利用正交实验分析影响木薯黄浆废水蛋白质沉淀因素主次和优化木薯黄浆废水蛋白质酸沉淀工艺,结果表明在pH为4.0、温度为35℃、提取时间为3.0h及蛋白质浓度为1400mg/L的条件下,木薯黄浆废水中蛋白质沉淀率达到最高为98.3%。木薯黄浆废水沉淀蛋白质富含15种氨基酸,可以作为动物饲料添加剂使用。Abstract: The major components of cassava starch maize plasm wastewater were analyzed to understand the subsequent processing and recycling of cassava starch wastewater. Isoelectric points of protein in the maize plasm cassava starch wastewater were between 3.5 to 4.5. Through the single factor experiment, pH, precipitation time, temperature and concentration of protein in cassava yellow pulp wastewater were selected as variables. The acid precipitation technology of protein in cassava starch maize plasm wastewater was studied by the orthogonal test. The results showed that the precipitation rate of protein were obtained reach as high as 98.3%, when the initial pH was 4.0, the temperature was 35℃, the precipitation time was 3.0h, and protein concentration for 1400mg/L. Cassava starch wastewater precipitated protein contained 15 kinds of amino acids, it could be used as animal feed additive.
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Keywords:
- cassava starch wastewater /
- isoelectric point /
- acid precipitation /
- orthogonal test
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