Study on the preparation and characterization of bioactive peptides from egg yolk
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摘要: 本实验采用乙醇-正己烷脱脂蛋黄粉为底物,Alcalase 2.4L为水解酶制剂,经两次酶解制备得一种蛋黄肽产品,产品得率达到41%。对该产品的基本物化指标进行了测定,蛋白含量高于85%,灰分含量低于10%,在较宽的pH范围内溶解性良好,热稳定性高。体外活性实验表明,该产品可以通过与钙离子结合抑制磷酸钙沉淀,表现出了潜在的促进肠道钙吸收的能力,且具有较好的抗氧化能力。Abstract: Employing defatted yolk power by ethanol-hexane extraction as substrate, yolk peptide with high quality was obtained by hydrolysis with 2.4L Alcalase.The hydrolysis process was conducted for twice and yield rate was up to 41%.The physical and chemical indexes of the product were measured: the peptide content was above 85%and the ash content was less than 10%, which features good solubility and high heat stability within a wide pH range. Activity experiment in vitro indicated that yolk peptide product had strong ability to prevent calcium phosphate from sediment, suggesting a potential on improving intracellular calcium absorption. Meanwhile, the oxidation resistance activity of yolk peptide was conformed in this study.
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Keywords:
- yolk powder /
- yolk peptide /
- antioxidant /
- Calcium-binding
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[1] McCann KB, Shiell BJ, Michalski WP, et al.Isolation and characterization of antibacterial peptides derived from the f (164-207) region of bovineαs2-casein[J].International Dairy Journal, 2005, 15:113-143.
[2] Korhonen H, Pihlanto A.Bioactive peptides:production and functionality[J].International Dairy Journal, 2006, 16:549-560.
[3] 徐彩娜, 殷涌光, 王二雷, 等.卵黄高磷蛋白及其磷酸肽的研究进展[J].食品工业科技, 2010, 31 (9) :428-451. [4] 徐彩娜, 林松毅, 刘静波, 等.双酶分步水解制备卵黄高磷蛋白磷酸肽的实验研究[J].食品科技, 2008, 11:6-10. [5] 张维农, 刘大川, 胡小泓.正己烷-含水乙醇混合溶剂制取大豆浓缩蛋白工艺的研究[J].中国油脂, 2002, 04:42-44. [6] Nissen JA.Enzymic Hydrolysis of Food Proteins[M].Elsevier Applied Science Publishers, 1986:132-152.
[7] GB/T 5009.4-2010食品中灰分的测定[S]. [8] GB/T5009.5-2010食品中蛋白质的测定[S]. [9] 马文, 赵亮, 谢复新, 等.钼蓝法测定玉米浆中磷含量[J].安徽大学学报, 1996, 01:31-33. [10] 蔡武城, 袁厚积.生物物质常用化学分析法[M].北京:科学出版社, 1982, 93. [11] 刘小丽.卵黄高磷蛋白及其磷酸肽的制备和研究[D].西安:西北大学, 2003. [12] Mina MY, Ahmad A, JamshidKhan C.A novel antioxidant and antimicrobial peptide from hen egg white lysozyme hydrolyzates[J].Journal of Functional Food, 2012 (4) :278-286.
[13] GB/T 22492-2008大豆肽粉[S]. [14] 陈历俊.大豆活性肽的研制与应用[D].哈尔滨:东北农业大学, 1998. [15] Li Y, TomeD, Desjeux JF.Indirect effect of casein phosphopeptides on calcium absorption in rat ileum in vitro[J].Reproduction, Nutrition, Development, 1989, 29:227-233.
[16] Hsu CL, Chen WL, Weng YM, et al.Chemical composition, physical properties, and antioxidant activities of yam flours as affected by different drying methods[J].Food Chemistry, 2003, 83:85-92.
[17] Ishikawa S, Yano Y, Arihara K, et al.Egg yolk phosvitin inhibits hydroxyl radical formation from the Fenton reaction[J].Bioscience, Biotechnology, and Biochemistry, 2004, 68:1324-1331.
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