Abstract: This study was designed to optimize process parameters of tomato juice. Based on the single factor experiments, the optimum process parameters conditions were determined by response surface methodology to be 0.08% cellulase added, enzymolysis time 79 min, pH4.0 and 54℃. Under the optimum conditions, the juice yield was 90.08%, which was consistent with the predicted yield of tomato. Thus, the fitted quadratic regression model was valid.