Optimization of deproteinized process with trichloroacetic acid method from polysaccharides of pholiota nameko by response surface methodology
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摘要: 实验优化了滑子菇粗多糖脱除蛋白质的工艺条件。采用响应曲面法对三氯乙酸从滑子菇多糖中脱除蛋白质的工艺进行研究,在单因素实验基础上,以多糖保留率和蛋白质脱除率为响应值,应用Box-Behnken中心组合实验方法进行三因素三水平的实验设计,通过响应面分析得到优化组合条件。结果表明:最优工艺参数为:料液比为1∶3,脱除时间为50min,三氯乙酸浓度为6%,在此工艺条件下,多糖保留率预测值为75.40%,实测值75.08%;蛋白质脱除率预测值为84.39%,实测值为84.16%,实测结果与预测值符合良好。Abstract: To optimize the deproteinization conditions from crude polysaccharide of pholiota nameko in order to improve reduction efficiency. The technology of removal of proteins frome nameko polysaccharides by richloroacetic acid method was optimized using response surface methodology (RSM) . On the basis of single factor experiments. The polysaccharide retention yield and the removal protein yield were used as the responsive values. Three-variable and three-level experiments design were developed by Box-Benhnken central composite design method. The optimized process conditions was obtained by the method of response surface analysis. Results indicated that the optimum technological conditions were as follows:the ratio of material to liquid 1∶3, the time of removing protein 50 min, the best concentration of trichloroacetic acid (TCA) 6%. Under the optimal conditions:the predicted and experimental retention yields of polysaccharides were75.40% and 75.08%, the predicted and experimental removal yields of proteins were 84.39% and 84.16%.Therefore, there was a good accordance between the predicted value and observed value.
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