Research of mutagenicity of the Cassava leaves
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摘要: 目的:研究木薯叶的致突变作用,为其安全利用提供参考。方法:蚕豆根尖微核试验、小鼠骨髓细胞微核试验及小鼠精子畸形试验。结果:木薯叶浓度为15.80mg/mL以上,蚕豆根尖微核率增加,与阴性对照相比较具有显著性差异(p<0.01),微核指数为1.77;小鼠骨髓微核率增加,与阴性对照相比存在显著性差异(p<0.05,p<0.01);木薯叶浓度为7.90mg/mL以上,对雄性小鼠的精子畸形率增加,与阴性对照比较存在显著性差异(p<0.01)。结论:一定浓度的木薯叶可能对植物细胞、动物体细胞和生殖均存在致突变作用。
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关键词:
- 木薯叶; /
- 毒理学安全性评价试验; /
- 致突变性;
Abstract: Objective: The mutagenic of cassava leaves was researched for its safe to use. Method: The relevant toxicology tests, including broad bean root tip micronucleus test /the micronucleus test of bone marrow cells in mice and sperm abnormality test in mice, were used to study the cassava leaves' s poisonous effect.Result: It was found that the broad bean root tip micronucleus rate would increase when the concentration of cassava leaves was above 15.80mg /mL, the micronucleus index was 1.77, and there was a significant difference between the group of experiment and the negative control ( p < 0.01) . It was discovered that the male and female mice bone marrow micronucleus rate was increased significantly when the concentration of cassava leaves was above 15.80mg /mL, compared with the negative control ( p < 0.05, p < 0.01) . When the concentration of cassava leaves was above7.90mg /mL, it was found that the male mice sperm malformation rate would increase, compared with negative control ( p < 0.01) .Conclusion: Deciding from this, a certain concentration of cassava leaves may have some harmful effect on the broad bean root tip cells /somatic cells and reproductive cells in mice. -
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