Extraction method of the oils and fats and the peroxide value determination in barbecue products
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摘要: 以烤肉制品为原料,采用正己烷-异丙醇溶剂浸提法提取油脂,旨在提高低脂肪烤肉制品中油脂提取率同时准确测定其过氧化值。油脂提取的最优条件:称取肉样（绞碎混匀）2.000g,加入正己烷-异丙醇（4∶1,v/v）混合溶剂20mL,浸泡过夜,2500r/min离心20min;旋转蒸发至尽干,于真空干燥箱中干燥1.5h。用该方法对不同烤肉制品进行验证性实验,均获得了较好的提取效果。该提取方法回收率为84.51%113.20%,提取率的相对标准偏差为0.12%0.67%,表明该油脂提取方法准确度和精密度均较高。测定所提油脂的过氧化值,结果表明该油脂提取方法不会影响其过氧化值测定结果,并解决了石油醚浸提法无法准确测定低脂肪肉制品（鸡肉）中过氧化值的问题。Abstract: Oil was extracted from barbecue products by n- hexane- isopropanol ( 4 ∶ 1, v /v) solvent extraction method, in order to improve the oil extraction rate in low- fat barbecue products and the degree of accuracy of peroxide value determination results in barbecue products.The results showed that the optimal conditions of oil extraction in barbecue products were determined to be the minced meat samples 2.000g were soaked in 20mL n-hexane- isopropanol ( 4∶1, v /v) for the night, and then centrifuged for 20min at the speed of 2500r /min, and the solvent of the oil extraction was removed with a rotary evaporator, and then dried for 1.5h in a vacuum oven.When it was used to extract the oil from the different barbecue products as validation experiments, a good extraction result was gained. The recovery of this method was in the range of 84.51% to 113.20%, and the relative standard deviation of extraction rate was in the range of 0.12% to 0.67%.This method has high accuracy and precision.It did not affect the peroxide value determination, and it can accurately measure the peroxide value in low- fat meat products, such as chicken.
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Keywords:
- barbecue products; /
- oils and fats; /
- the extraction agent; /
- the extraction rate;
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