Effects of condensed fish oil on the growth of prostate cells in vitro
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摘要: 探讨了鱼油对前列腺正常细胞（RWPE-1）和前列腺癌细胞（PC-3）生长和代谢的影响。经鱼油处理,MTT法及流式细胞术考察鱼油对以上两细胞生长的影响,气相色谱法分析处理前后脂肪酸组成,酶联免疫法检测细胞炎性因子（TNF-α,IL-6,LXA4）的表达。结果显示,本实验所用浓缩鱼油基本不影响前列腺正常细胞RWPE-1的生长,而50倍稀释鱼油作用48h可使PC-3细胞存活率降至70.34%,凋亡率上升至15.47%±0.64%;鱼油作用后PC-3细胞内γ-亚麻酸、花生四烯酸、EPA和DHA比例明显增加,而在RWPE-1细胞内并无明显增加;RWPE-1细胞的LXA4表达量基本不变,IL-6和TNF-α表达量逐渐上升,而PC-3细胞的LXA4表达量呈明显上升趋势,IL-6、TNF-α的表达量却显著下降。本实验所用浓缩鱼油对RWPE-1和PC-3细胞的生长、代谢具有不同的影响。Abstract: In order to elucidate the effects of fish oil on the growth and metabolism of prostate cells, in the present paper an epithelial cell (RWPE-1) and prostate cancer cell (PC-3) were employed. Effects of fish oil on cell growth and apoptosis were investigated by MTT assay and flow cytometry after treatment with fish oil.Also gas chromatography was used to analyse fatty acids composition and inflammatory factors (TNF-α, IL-6, LXA4 secreted by cells were detected using ELISA. Results showed that condensed fish oil used in our study had little damage to RWPE-1 cell growth, but the viability of PC-3 cells reduces to 70.34% and the apoptosis rate of PC-3 cells is up to 15.47%±0.64% after treated with fish oil for 48 h which was diluted into 50 times. The proportions of GLA, AA, EPA and DHA in PC-3 cells increased markedly, which had no significant increase in RWPE-1 cells. For RWPE-1 cells, the secreted LXA4 maintains almost in a same level and the expression of IL-6 and TNF-α rises gradually. However, PC-3 cells resulted in higher levels of LXA4 and lower expressions of IL-6 and TNF-α when incubated with fish oil. Effects of condensed fish oil used in our study on the growth of these two kinds of prostate cells are quite different.
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Keywords:
- fish oil /
- prostate cancer /
- cell apoptosis /
- fatty acids composition /
- inflammatory factors
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