Antibacterial activities of Xanthan Oligosaccharides against Xanthomonas campestris
-
摘要: 碱性条件下对黄原胶进行氧化降解,透析得到三种黄原胶寡糖（XGO-A,XGO-B和XGO-C）,对产物进行FT-IR表征,GPC法测定其分子量分别为3600、7500和10100。三种黄原胶寡糖的丙酮酸含量分别为5.7%、5.3%和4.8%;还原糖含量分别为23.7%、22.5%和21.1%。采用琼脂平板打孔法、最低抑菌浓度以及生长曲线方法,考察了三种黄原胶寡糖对野油菜黄单胞菌的抑菌能力;同时检测了三种寡糖对野油菜黄单胞菌细胞膜通透性的影响。结果表明,分子量为7500的XGO-B具有更好的抑菌性能。而当丙酮酸和还原糖含量相近时,对野油菜黄单胞菌的抑菌性能可能与黄原胶寡糖的分子量有关;随着分子量的上升,寡糖对野油菜黄单胞菌细胞膜破坏增强,这表明破坏细胞膜通透性是抑菌机理之一。Abstract: Three xanthan oligosaccharides ( XGO-A, XGO-B and XGO-C) were prepared by oxidative degradation in alkaline condition.The products were characterized by FT- IR, and the molecular weights of the products determined by GPC method were 3600, 7500 and 10100, respectively.The pyruvate acid contents of the three xanthan oligosaccharides were 5.7%, 5.3% and 4.8%, respectively, and reducing sugar contents were 23.7%, 22.5% and 21.1%, respectively.The antibacterial activity of three xanthan oligosaccharides against the X.campestris was investigated by the inhibition zone, minimal inhibitory concentration ( MIC) and the influence on the growth of X. campestris.Effects of xanthan oligosaccharides on electric conductivity of liquid medium of X.campestris were also determined.The results indicated that XGO-B exhibited higher antibacterial activity than XGO-A and XGO-C. It meant that the antibacterial effect of xanthan oligosaccharides may be related to their molecular weight.The effect of xanthan oligosaccharides on the cell membrane permeability of X.campestris became stronger when the molecular weight of xanthan oligosaccharides increased.It indicated that the damage of cell membrane of X.campestris is one of the antibacterial mechanisms to X.campestris.
-
Keywords:
- xanthan /
- oxidative degradation /
- antibacterial activity
-
[1] 黄成栋, 白雪芳, 杜昱光.黄原胶 (Xanthan Gum) 的特性、生产及应用[J].微生物学通报, 2005, 32 (2) :91-98. [2] 郭瑞, 丁恩勇.黄原胶的结构、性能与应用[J].日用化学工业, 2006, 36 (1) :42-45. [3] 韩冠英, 凌沛学, 王凤山.黄原胶的特性及其在医学领域的应用[J].生物医学工程研究, 2010, 29 (4) :277-281. [4] 钱方.黄原胶生物降解及其寡糖生理活性的研究[D].大连:大连理工大学, 2008. [5] Qian F, An LJ, He XY, et al.Antibacterial activity of xanthan-oligosaccharide cleaced from xanthan against phytopathogenic Xanthomonas campestris pv.campestris[J].Process Biochemistry, 2006, 41:1582-1588.
[6] Xiong XY, Li M, Xie J, et al.Antioxidant activity of xanthan oligosaccharides prepared by different degradation methods[J].Carbohydrate Polymers, 2013, 92:1166-1171.
[7] 江丽丽, 张庆, 徐世艾.黄原胶降解的研究进展[J].微生物学通报, 2008, 35 (8) :1292-1296. [8] 孙涛, 熊小英, 魏颖隽, 等.黄原胶降解及其抗氧化性能研究[J].天然产物研究与开发, 2012, 24:102-104, 113. [9] 楼良旺, 高年发.紫外分光光度法测定丙酮酸[J].分析实验室, 2005, 24 (4) :11-13. [10] Miller G L.Use of dinitrosalicylic acid reagent for determination of reducing sugar[J].Analytical Chemistry, 1959, 31:426-428.
[11] Fernandes JC, Tavaria FK.Antimicrobial effect of chitosans and chitooligosaccharides, upon Staphylococcus aureus and Escherichia coli, in food model systems[J].Food Microbiology, 2009, 25:922-928.
[12] Jeon YJ.Antimicrobial effect of chitooligosaccharides produced by bioreactor[J].Carbohydrate Polymers, 2001, 44:71-76.
[13] Hong KN, Na YP, Shin HL, et al.Antibacterial activity of chitosan and chitosan oligomers with different molecular weights[J].International Journal of Food Microbiology, 2002, 74:65-72.
[14] 侯伟峰, 谢晶, 蓝蔚青, 等.植酸对大肠杆菌抑菌机理的研究[J].江苏农业学报, 2012, 28 (2) :443-447. [15] 朱淮武.有机分子结构波谱解析[M].北京:化工出版社, 2008:50-51. [16] Madigan M T, Martinko J M.微生物生物学[M].11版.李明春, 杨文博, 译.北京:科学出版社, 2009, 95-113. [17] 何晓燕.黄原胶寡糖生物活性的研究[D].大连:大连轻工业学院, 2005.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
