Antioxidant capacity of phenolic extracts from Sphallerocarpus gracilis stem leaves
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摘要: 为研究黄参茎叶中酚类提取物的抗氧化活性,本文以分光光度法测定黄参茎叶中酚类提取物的总还原力、亚铁离子螯合能力、对β-胡萝卜素/亚油酸自氧化体系的抑制作用、对亚硝酸盐的清除作用,及对DPPH自由基（DPPH·）、羟基自由基（·OH）、超氧阴离子（O2-·）的清除作用,并与人工合成的抗氧化剂2,6-二叔丁基-4-甲基苯酚（BHT）和天然抗氧化剂VC进行对比。结果表明:黄参茎叶多酚具有较好的体外抗氧化性能,部分指标甚至优于阳性对照;相同浓度下黄参茎叶多酚粗提物和纯化物在不同测定体系中的清除能力有显著性差异（p<0.05）。黄参茎叶中酚类提取物可作为天然抗氧化剂进一步开发和利用。Abstract: The antioxidant activity of phenolic extracts from Sphallerocarpus gracilis stem leaves was studied.The antioxidant activity of the extract was evaluated by spectrophotometry on its ability of scavenging 1-diphenyl-2-picrylhydrazy (DPPH·) , hydroxyl radical (·OH) , superoxide anion radical (O2-·) , nitrite, β-carotene bleaching test, and total reducing power. BHT and VC were used as positive control. Results indicated that the polyphenol extracts had a good antioxidant capacity, some indicators even better than the positive control. In addition, polyphenol extracts and purified in a different measurement system in the scavenging ability were quite different at the same concentration (p<0.05) . It could be used as natural antioxidants further developed and utilized.
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Keywords:
- Sphallerocarpus gracilis /
- stem leaves /
- phenolic extracts /
- antioxidant
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