Purification of polyphenols from Korean pine cone lamella by SephadexLH-20 and their antioxidative activities in vitro
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摘要: 为了得到高纯度红松松球鳞片多酚样品和初步评价其抗氧化活性,采用葡萄糖凝胶LH-20对红松松球鳞片多酚的初级纯化物进行二次精制,得到了纯度为95.86%的多酚样品。该多酚样品对DPPH自由基、ABTS自由基和羟自由基具有良好的清除作用,IC50分别为20.48、86.59μg/mL和0.45mg/mL;对铁离子（Fe3+）和铜离子（Cu2+）具有很强的还原能力,其还原铁离子（Fe3+）能力与V C相近,还原铜离子（Cu2+）能力略低于V C;对亚铁离子具有一定的螯合作用,其IC50为2.14mg/mL;对卵黄脂质过氧化具有明显的抑制作用,其IC50为0.33mg/mL。
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关键词:
- 红松松球鳞片 /
- 多酚 /
- 葡萄糖凝胶LH-20 /
- 体外抗氧化
Abstract: A polyphenols-rich extract from Korean pine cone lamella after the preliminary purification was further purified by Sephadex LH-20 gel chromatography, and high purified polyphenol ( HPP) which purity reached 95.86% was obtained. In order to estimate the capacity of HPP to act as antioxidants, its DPPH radical, ABTS radical, hydroxyl radical scavenging activity, ferric ions ( Fe3 +) and cupric ions ( Cu2 +) reducing ability, ferrous ions ( Fe2 +) chelating activity, yelk peroxidation inhibiting effect were studied. HPP was found to exhibit remarkable scavenging activity on DPPH· ( IC 50 20.48μg /mL) , ABTS+· ( IC 50 86.59μg /mL) , OH· ( IC 50 0.45mg /mL) , as well as a high and dose-dependent Fe3 +, Cu2 +reducing power. Moreover, it possessed good chelating activity ( IC 50 2.14mg /mL) and yelk peroxidation inhibiting effect ( IC 50 0.33mg /mL) . -
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