Study on extraction for chlorophyll from filter mud in sugar cane mill factories by enzymatic hydrolysis
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摘要: 以甘蔗糖厂滤泥为材料,用体积分数95%的酒精为提取溶剂,研究添加纤维素酶和果胶酶提取叶绿素的实验。在单因素实验基础上,选取混合酶酶解温度、混合酶酶解时间、料液比为自变量,叶绿素的提取量为响应值,根据BoxBehnken中心组合实验设计原理采用三因素三水平的响应面分析法,研究各自变量及其交互作用对叶绿素提取量的影响,模拟得到二次多项式回归方程的预测模型。结果表明,果胶酶和纤维素酶混合使用比单一酶的提取效率高,叶绿素的最优提取工艺参数为:纤维素酶和果胶酶混合质量比2∶1,添加混合酶总量1.6g/100g,混合酶酶解温度49℃,酶解时间3h,料液比1∶8(g/mL)。在此条件下叶绿素的提取量为0.211mg/g,比传统溶剂提取法高出8%。Abstract: In the extraction of chlorophyll from filter mud in sugar cane mill factories, volume fraction of 95% alcohol was used as extraction solvent.Chlorophyll extraction was investigated with respect to the effects of respective and synergic hydrolysis of filter mud by cellulase and pectinase. Three extraction parameters including enzymolysis temperature of mixed enzyme, enzymolysis time of mixed enzyme and ratio of material to liquid were optimized using central composite design and response surface methodology based on single factor investigations for achieving maximum the chlorophyll extraction quantity.The interaction of the respective variables and their influence on the chlorophyll extraction yield was studied by using box- behnken central composite design and response surface analysis theory, the simulated quadratic polynomial regression equation of prediction model was set up.The results indicated that more chlorophyll was extracted from filter mud by using both of the enzymes than using cellulase alone, the optimal conditions for extraction of chlorophyll were cellulase /pectinase ratio 2 ∶ 1, their total dosage 1.6g /100 g, enzymolysis temperature of 49℃, enzymolysis time of 3h and material to liquid ratio of1∶8 ( g /mL) . Under these conditions, the extraction yield of chlorophyll was 0.211mg·g- 1. Compared with the traditional extraction, enzymatic hydrolysis extraction revealed an enhanced yield of chlorophyll by 8%.
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