Optimization of extraction conditions of total flavonoids from Zizyphus jujube by quadratic regression orthogonal rotating combination
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摘要: 本文以红枣为原料,以总黄酮的提取率和DPPH·清除率为考察指标,研究提取温度、提取剂浓度、料液比、提取时间、加酶量、pH、超声功率对提取率及抗氧化能力的影响;并通过三元二次回归正交旋转设计对提取条件进行优化,建立了单因素与提取率和DPPH·清除率的回归方程,确定超声波酶辅助法的最佳提取条件为:温度45℃,提取剂浓度60%,料液比1∶25,提取时间50min,加酶量0.6%,pH4,超声功率180W。最佳提取条件下总黄酮的提取率为0.537%,此时DPPH·清除率为40.2%。Abstract: In this study, zizyphus jujube was used as the raw material to investigate the clearance rate of DPPH·and the extraction rate of flavonoids by ultrasonic treatment. Effects of extraction temperature, extractant concentration, solid- liquid ratio, extraction time, enzyme dose, pH value, ultrasonic power on preparation of flavonoids with cellulase were studied by single factor analysis. The regression models were established with factors and the indexes, including extraction efficiency and DPPH· clearance rate.The results showed that the optimum values of temperature, extractant concentration, solid- liquid ratio, extraction time, enzyme dosage, pH and ultrasonic power were 45℃, 60%, 1∶25, 50min, 0.6%, 4 and 180W.Under this condition, the extraction efficiency was 0.537%, the DPPH·clearance rate was 40.2 %.
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Keywords:
- zizyphus jujube /
- cellulase /
- flavonoids /
- DPPH·clearance rate
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