Optimization of heterotrophic culture conditions of Chlorella sp. for lutein production
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摘要: 研究了异养条件下不同碳源、氮源、pH和温度等因素对一株自分离小球藻的生长及叶黄素产量的影响。结果表明,葡萄糖和硝酸钾分别是支持这株小球藻持续快速生长生产叶黄素的最佳碳源和氮源。异养培养小球藻生产叶黄素的最佳条件为:葡萄糖浓度20g·L-1、硝酸钾浓度1.25g·L-1,初始pH6.0,温度30℃,叶黄素产量为12.27mg·L-1。Abstract: The effect of carbon source, nitrogen source, pH as well as temperature on the growth of a self-isolated Chlorella sp. and the production of lutein were investigated. Results showed that glucose was the best carbon source and potassium nitrate was the best nitrogen source to support sustained and rapid growth of this strain of Chlorella sp. as well as lutein production. Results of optimization were as follows:the glucose concentration was 20g·L-1, potassium nitrate concentration was 1.25g·L-1, initial pH was 6.0 and temperature was 30℃. In this condition the maximal lutein concentration was 12.27mg·L-1.
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Keywords:
- Chlorella sp. /
- heterotrophic culture /
- lutein /
- carbon source /
- nitrogen source
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