雅致小克银汉霉的复合酶破壁工艺研究

详细信息

Study on cell disruption of complex enzyme of Cunninghamella elegans

College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology
National Biochemical Center of Nanjing University of Technology

摘要: 利用复合酶对雅致小克银汉霉进行破壁工艺研究,通过研究复合酶添加量、酶解pH、酶解温度、酶解时间等因素对破壁效果的影响,确定较优的工艺条件为:复合酶添加量为0.3%、酶解pH为9、酶解温度为50℃、酶解时间为4h,最终提取率为87.3%,可为相应的工业化作参考。
- 复合酶 /
- 细胞破壁 /
- γ-亚麻酸
Abstract: Using the complex enzyme to break the cell of Cunninghamella elegant, the optimum process conditions were determined by studying the addition of enzyme, pH, temperature, reaction time. The optimum process conditions were as followed: enzyme dosage 0.3%, hydrolysis pH9, hydrolysis temperature 50℃, reaction time 4h, the final extraction rate reached 87.3%. The final process can serve as a reference for the corresponding industrialization.
- complex enzyme /
- cell broken /
- γ-linolenic

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