Study on bactericidal effect of chlorine dioxide gas to bacteria on the surface of wheat and strain identification
-
摘要: 研究了不同浓度的气体二氧化氯对小麦表面细菌的杀菌效果。结果表明,气体二氧化氯对小麦防霉处理的最佳条件为:杀菌时间90min,杀菌浓度为8mg/L时,杀菌率可达99.90%;在上述条件下对不同含水率的小麦进行杀菌,含水率在17%以下时,杀菌率可达99%以上;对小麦表面菌落进行革兰氏染色和芽孢染色,鉴定结果表明,小麦表面的菌落,主要为革兰氏阳性菌和革兰氏阴性菌,没有芽孢生长。Abstract: Gas chlorine dioxide of different concentrations was applied to the surface sterilization of wheat. The results showed when the optimal condition of gas chlorine dioxide on wheat mildewproof treatment was sterilization time of 90min, bactericidal concentration of 8mg /L, the sterilization rate reached 99.90%.Under these conditions, different moisture content of wheat was sterilized, and when moisture content was less than 17%, the sterilization rate could be up to 99%.Gram staining and spore staining was conducted on the surface colonies of wheat.The results showed that the main colonies on the surface of wheat were gram-positive bacterium and gram-negative bacterium, without spore growth.
-
Keywords:
- chlorine dioxide gas /
- wheat /
- sterilization /
- strain identification
-
[1] 程芳.小麦和玉米储藏期间微生物多样性变化及控制[D].济南:山东农业大学食品科学与工程学院, 2012. [2] 杨学明, 姚金宝, 马鸿翔, 等.杀菌剂对弱筋小麦9号品质和产量的影响[J].江苏农业学报, 2012, 28 (4) :709-712. [3] 胡文忠, 姜爱丽, 田中俊一郎.红外线照射谷物表面杀菌的研究[J].食品与机械, 2007, 23 (4) :8-11. [4] Hamanaka D, Dokan S, Yasunaga E, et al.The sterilization effects of infrared ray on the agricultural products spoirage microorganisms.2000 ASAE Annual International Meeting, Technical Papers:Engineering Solutions for a New Century[C].2000:971-979.
[5] 晏晖.三唑酮、福美双复配包衣对春小麦病害的作用效果[J].种子, 2002, 3:35-36. [6] Ri Ya Jin, Shuang Qi Hu, Ying Hao Zhang, et al.Research on the explosion characteristics of chlorine dioxide gas[J].Chinese Chemical Letters, 2008, 19:1375-1378.
[7] 韦明肯, 赖洁玲, 詹萍.二氧化氯杀菌机理研究进展[J].微生物学报, 2012, 52 (4) :429-434. [8] 晋日亚, 胡双启.气体二氧化氯对苹果表面细菌杀菌规律研究[J].食品科学, 2008, 29 (7) :109-112. [9] 申小静, 胡双启.气体二氧化氯对鲜枣表面青霉菌的杀灭效果研究[J].食品工业科技, 2010, 31 (6) :301-302. [10] 奚小艳.气体二氧化氯对公共场所空间环境消毒效果研究[D].太原:中北大学化工与环境学院, 2012. [11] GB_4789.2-2010, 食品卫生微生物学检验菌落总数测定[S]. [12] 周群英, 王士芬.环境工程微生物学[M].北京:高等教育出版社出版, 2008. [13] 崔超, 胡双启, 晋日亚, 等.气体二氧化氯的光解规律研究[J].中国安全科学学报, 2011, 21 (7) :53-55. [14] 张克平, 黄建龙, 杨敏, 等.冬小麦籽粒受挤压特性的有限元分析及实验验证[J].农业工程学报, 2010, 26 (6) :352-355. [15] 洪庆华, 迟杰, 齐云, 等.影响革兰氏染色结果的原因分析[J].实验技术与管理, 2011, 28 (5) :41-43. [16] 张俊会, 马国芳.光学显微镜下细菌芽孢染色方法的探讨[J].生物学杂志, 2011, 28 (6) :106-108.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
