Protective effect of buckwheat bee pollen on oxidative stresses and their component analysis
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摘要: 本文研究了荞麦蜂花粉的醇提取物和水提取物,测定了其体外抗氧化能力及其对骨髓干细胞增殖的促进能力,据此筛选出功能性更强的荞麦蜂花粉水提取物,研究其对骨髓干细胞的辐射防护作用,并测定了其氨基酸和多糖的组成。结果表明:荞麦蜂花粉水提取物及醇提取物均具有一定的抗氧化能力,且呈剂量依赖关系。荞麦蜂花粉水提取物对骨髓干细胞促增殖活性最高可达74.74%,其促进作用显著高于醇提取物。荞麦蜂花粉水提取物主要成分为糖及蛋白质,其富含多种氨基酸,其中谷氨酸含量最高,其次为天冬氨酸、赖氨酸、苏氨酸及丝氨酸。荞麦蜂花粉水提取物的单糖组成主要为核糖、鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖及半乳糖,其物质的量比为0.35∶0.95∶27.78∶0.93∶1.70∶13.19∶16.16。Abstract: The present study was to investigate buckwheat bee pollen in terms of its antioxidant capacities and its ability to promote the proliferation of bone marrow stem cells.The water extract of buckwheat bee pollen ( WEBBP) performed better than its ethanol extract in radical scavenging and stem cell pro- proliferation. Afterwards the protective effect of WEBBP on irradiated bone marrow stem cells were studied and its components were determined.The results showed that both water and alcohol extracts of buckwheat bee pollen showed dose- dependent antioxidant abilities.WEBBP demonstrated a better capacity in promoting stem cell proliferation ( 74.74%) than its alcohol extract.The main components of WEBBP were carbohydrate and protein.In addition, it had varieties of amino acids, with glutamate being the highest in content, followed by aspartate, lysine, threonine and serine. The monosaccharide components of WEBBP's carbohydrates were measured. They concluded ribose, rhamnose, arabinose, xylose, mannose, glucose and galactose, with a molar ratio of 0.35∶0.95∶27.78∶0.93∶1.70∶13.19∶16.16.
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Keywords:
- buckwheat bee pollen /
- bone marrow stem cells /
- proliferation /
- antioxidant /
- radio-protective
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