Effect of different drying methods on the active compounds and browning enzyme activities from the Bud of Lonicerae Confusae
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摘要: 目的:研究不同干燥方法对山银花酶活性及相关活性成分含量的影响,为确定山银花最佳干燥方法,提高山银花品质提供实验依据。方法:采用真空微波干燥、恒温鼓风干燥、阴干对山银花样品进行干燥处理;利用分光光度计法测定样品多酚氧化酶、过氧化物酶的酶活性以及总酚、总黄酮含量;高效液相色谱法测定绿原酸及木犀草苷含量。结果:真空微波处理的山银花样品,多酚氧化酶及过氧化物酶酶活性最低,剩余酶活性仅为鲜样的30%和13%,产生轻微褐变,总酚、总黄酮、绿原酸、木犀草苷含量最高;阴干样品大部分酶活性仍保留,褐变严重,活性成分含量最低。结论:不同干燥方法对山银花酶活性及活性成分有较大影响,真空微波干燥为最佳干燥方法。Abstract: Objective: To choose the best drying method and improve the quality of Lonicerae Confusae, the effect of different drying methods on the enzyme activities and the contents of active compounds from the bud of Lonicerae Confusae was studied. Method: Dehydrating methods of vacuum microwave drying, air blast drying and shade drying were chosen to deal with buds of Lonicerae Confusae. The enzyme activity and the content of total phenoltotal, total flavonoids were determined by spectrophotometer. The contents of chlorogenic acid and luteoloside from different drying methods were determined by HPLC.Results: Using the vacuum microwave drying method, the activities of polyphenol oxidase and peroxidase showed a minimum value, the residual activity remained only 30% and 13% resulting in a slight browning. Total phenols, total flavonoids, chlorogenic acid and luteoloside were all the highest compared with other two drying methods.The residual enzyme activity still remained above 80% dried by shade drying method, the browning happened seriously, the content of active compounds was the least.Conclusion: The different drying methods had greater impact on the active compounds and enzyme activities, the vacuum microwave drying was the best method.
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Keywords:
- Lonicerae Confusae /
- drying methods /
- enzyme activity /
- active components
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