Study on antioxidant activity of guava polyphenols in vitro
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摘要: 微波辅助提取的番石榴多酚粗提物经过NKA-9大孔吸附树脂初步纯化和Sephadex LH-20葡聚糖凝胶的分离,得到四个不同组分（GP1、GP2、GP3、GP4）。以抗坏血酸（V C）为阳性对照,采用总抗氧化能力、羟自由基、超氧阴离子自由基和DPPH自由基四种体外抗氧化能力评价体系测定了番石榴粗多酚（CGP）、大孔吸附树脂纯化多酚（PGP）和各分离组分的抗氧化能力。结果表明:番石榴多酚具有很高的抗氧化活性,总抗氧化能力大小为GP4>V C>GP3>GP2>PGP>CGP>GP1。番石榴多酚能有效地清除羟自由基、超氧阴离子自由基和DPPH自由基,在一定范围内清除自由基能力和浓度成线性关系。Abstract: The crude guava polyphenols was extracted by the microware assisted extraction, and then purified with NKA-9 macro porous resin. After the separation of purified guava polyphenols with the Sephadex LH-20, four different components were obtained (GP1, GP2, GP3 and GP4) . The total antioxidant activity (T-AOC) , DPPH radical scavenging, hydroxyl radical scavenging and superoxide anion scavenging were selected to study the antioxidant activity in vitro of the crude guava polyphenols (CGP) , the purified guava polyphenols (PGP) and the four different components. Ascorbic acid (VC) was used as the positive control. The results showed that the GP4 had the strongest total antioxidant activity, followed by VC, GP3, GP2, PGP, CGP and GP1. And the guava concentration and free radical scavenging capacity was a linear relationship within a certain concentration range.
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Keywords:
- guava /
- polyphenols /
- separation /
- antioxidant activity
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