Detection of class Ⅰ integron from foodborne salmonella and analysis on the drug resistance gene cassettes
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摘要: 目的:了解食源性沙门氏菌的耐药性、Ⅰ类整合子分布及其耐药基因盒的结构序列,为Ⅰ类整合子的分子进化提供理论基础。方法:采用K-B纸片法检测食源性沙门氏菌对14种抗生素的敏感性;利用聚合酶链式反应（PCR）扩增整合酶基因intI1检测食源性沙门氏菌中Ⅰ类整合子携带率及可变区耐药基因盒。结果:食源性沙门氏菌对β-内酰胺类、氨基糖苷类、喹诺酮类和磺胺类的最高耐药率分别为18.75%、12.5%、6.25%和25%。59.4%（19/32）的食源性沙门氏菌检测出第Ⅰ类整合子。PCR扩增Ⅰ类整合子耐药基因盒,扩增产物大小为1009和1664bp,携带aadA5、dfr17和aadA2耐药基因,可分别介导对氨基糖苷类抗生素壮观霉素、链霉素和磺胺类药物甲氧苄氨嘧啶的耐药。结论:食源性沙门氏菌中也检测到较高的耐药率及整合子携带率,提示我们应该从基因水平上对食源性致病菌耐药及其传播进行监测。Abstract: Objective: To investigate the drug resistance, the distribution of class Ⅰ integron and drug resistance gene cassette of foodborne salmonella. Methods: K- B assay was applied to measure the drug resistance of foodborne salmonella isolated against fourteen antibiotics.The class Ⅰintegron and drug resistance gene cassette were detected by PCR sequencing of amplification products. Results: The highest drug resistance rate to β-lactams, aminoglycosides, quinonones and sulfa in foodborne salmonella was 18.75%, 12.5%, 6.25% and 25%. 59.4% ( 19 /32) of foodborne salmonella were positive for classⅠintegron.The product by PCR amplifying of classⅠ integron was 1009bp and 1664bp. In the drug resistance cassettes of variable regions of class Ⅰ integrons there were 3 types drug resistance genes, including aadA5, dfr17 and aadA2, which induced the resistance to aminoglycosides antibiotics spectinomycin, streptomycin and sulfonamides drugs trimethoprim. Conclusion: High multidrug resistance and class I integron carrying had been identified among the foodborne salmonella.The result stressed the need for continued surveillance of foodborne antibiotic resistance bacteria in the molecular level.
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Keywords:
- Salmonella /
- class Ⅰintegron /
- resistance gene cassette /
- drug resistance
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