Effect of pH and dialdehyde starch on the properties of tilapia skin gelatin films
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摘要: 考察了pH和双醛淀粉(DAS)对鱼皮明胶膜的机械性能和耐水性能的影响。结果表明,随着pH的升高,明胶膜的抗拉伸强度(TS)呈现先增加后降低的趋势,pH7时达到最大值(23.78MPa),而膜的固形物溶解率(MS)和蛋白溶解率(PS)逐渐下降。当明胶膜添加了DAS后,膜的TS显著增大(p<0.05),而MS和PS都显著下降(p<0.05)。另一方面,在pH7下,添加1.5%的DAS可有效提高膜的机械性能和耐水性能。SDS-PAGE分析结果表明,在成膜过程中pH对明胶蛋白组分没有产生明显的影响,DAS与明胶蛋白之间发生了交联反应。Abstract: The effect of pH and dialdehyde starch ( DAS) on the mechanical properties and water resistance of edible gelatin films prepared from tilapia skins was investigated. With increasing pH of film- forming solution, the tensile strength ( TS) of films firstly increased, but a decrease of TS was observed after pH7, where the maximum TS of 23.78MPa was found.However, the matter solubility ( MS) and protein solubility ( PS) of gelatin films decreased gradually with increasing pH.The TS of gelatin films increased when incorporating DAS into film- forming solution, while their MS and PS declined significantly.On the other hand, the mechanical properties and water resistance of films were effectively improved by adding 1.5% DAS at pH7.Based on the SDS-PAGE analysis, no obvious change in the gelatin components was found at different pH during the film forming process, and the crosslinking between gelatin proteins was mainly induced by DAS.
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Keywords:
- gelatin films /
- dialdehyde starch /
- mechanical properties /
- water resistance
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[1] 翁武银, 刘光明, 苏文金, 等.鱼皮明胶蛋白膜的制备及其热稳定性[J].水产学报, 2011, 35 (12) :1890-1896. [2] Ou S, Kwork KC, Kang Y.Changes in in vitro digestibility and available lysine of soy protein isolate after formation of film[J].Journal of Food Engineering, 2004, 64 (3) :301-305.
[3] 吴菲菲, 翁武银, 苏文金, 等.罗非鱼鱼鳞明胶蛋白膜的制备及特性[J].食品工业科技, 2013, 34 (11) :277-282. [4] Shiku Y, Hamaguchi PY, Tanaka M.Effect of pH on the preparation of edible films based on fish myofibrillar proteins[J].Fisheries Science, 2003, 69 (5) :1026-1032.
[5] Hoque MS, Benjak Dal S, Prodpran T.Properties of film from cuttlefish (Sepia pharaonis) skin gelatin incorporated with cinnamon, clove and star anise extracts[J].Food Hydrocolloids, 2011, 25 (5) :1085-1097.
[6] 郑学晶, 霍书浩.天然高分子材料[M].北京:化学工业出版社, 2010:167-168. [7] Cao N, Fu YH, He JH.Mechanical properties of gelatin films cross-linked, respectively, by ferulic acid and tannin acid[J].Food Hydrocolloids, 2007, 21 (4) :575-584.
[8] Bigi A, Cojazzi G, Panzavolta S, et al.Mechanical and thermal properties of gelatin films at different degrees of glutaraldehyde crosslinking[J].Biomaterials, 2001, 22 (8) :763-768.
[9] Carvalho De RA, Grosso CRF.Characterization of gelatin based films modified with transglutaminase, glyoxal and formaldehyde[J].Food Hydrocolloids, 2004, 18 (5) :717-726.
[10] Rhim JW, Gennadios A, Weller CL, et al.Soy protein isolate-dialdehyde starch films[J].Industrial Crops and Products, 1998, 8 (3) :195-203.
[11] 程庆甦, 王硕, 李秋鸿, 等.双醛淀粉/胶原共混膜的制备及性能研究[J].皮革科学与工程, 2010, 20 (6) :8-16. [12] 王保金.双醛淀粉及其交联效率的研究[D].广州:广东工业大学, 2004. [13] Schacht E, Bogdanov B, Bulcke AVD, et al.Hydrogels prepared by crosslinking of gelatin with dextran dialdehyde[J].Reactive and Functional Polymers, 1997, 33 (2-3) :109-116.
[14] Martucci JF, Ruseckaite RA.Tensile properties, barrier properties, and biodegradation in soil of compression-molded gelatin-dialdehyde starch films[J].Journal of Applied Polymer Science, 2009, 112 (4) :2166-2178.
[15] 郑学晶, 李俊伟, 刘捷, 等.双醛淀粉改性明胶膜的制备与性能研究[J].中国皮革, 2011, 40 (23) :28-36. [16] 陈书霖, 陶忠, 吴菲菲, 等.鱼皮明胶蛋白膜的制备及其性质改良[J].集美大学学报:自然科学版, 2012, 17 (5) :335-342. [17] Lowry OH, Rosebrough NJ, Farr AL, et al.Protein measurement with the folin phenol reagent[J].The Journal of Biological Chemistry, 1951, 193 (1) :265-275.
[18] Steel RGD, Torrie JH.Principles and procedures of statistics:a biometrical approach[M].New York:McGraw-Hill, 1980.
[19] Farris S, Song JH, Huang QR.Alternative reaction mechanism for the cross-linking of gelatin with glutaraldehyde[J].Journal of agricultural and food chemistry, 2010, 58 (2) :998-1003.
[20] Nayudemma Y, Joseph KT, Bose SM.Studies on the interaction of collagen with dialdehyde starch[J].Journal of the American leather chemists association, 1961, 56:548-567.
[21] Rhim JW, Gennadios A, Handa A, et al.Solubility, tensile, and color properties of modified soy protein isolate films[J].Journal of Agricultural and Food Chemistry, 2000, 48 (10) :4937-4941.
[22] Gennadios A, Handa A, Froning GW, et al.Physical properties of egg white-dialdehyde starch films[J].Journal of Agricultural and Food Chemistry, 1998, 46 (4) :1297-1302.
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