Study on extraction of corn germ protein by two-step enzymatic method
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摘要: 为确定更为温和、高效的玉米胚芽蛋白提取工艺,本实验采用纤维素酶和碱性蛋白酶对玉米胚芽进行两步酶法处理。通过单因素考察和正交实验设计对提取工艺参数进行优化。结果表明,纤维素酶酶解的最佳工艺为:pH4.5、酶解时间2.5h、加酶量0.3%(W/V);碱性蛋白酶酶解的最佳工艺为:pH9.0、酶解时间3h、加酶量4%(V/V)。在此条件下,玉米胚芽蛋白的提取率可达到83.7%±1.2%。Abstract: In order to investigate gentler and more efficient corn germ protein extraction method, the corn germ protein was extracted from corn germ by the two-step enzymatic method ( cellulase and alkaline protease) .Single factor experiments and orthogonal experiments were performed to optimize the extraction processing parameters. The results showed that, the optimum conditions for cellulase processing were as follows: pH4.5, reaction time 2.5h and enzyme dosage 0.3% ( W/V) .The optimum conditions of alkaline protease processing were as follows: pH 9.0, reaction time 3h and enzyme dosage 4% ( V/V) . Under the optimum conditions, the extraction rate of corn germ protein was 83.7% ± 1.2%.
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Keywords:
- corn germ protein /
- cellulose /
- alkaline protease /
- extraction
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[1] Kulakova E V, Vainerman E S, Rogoshin S V.Contribution to the investigation of the corn germ Part I.Corn germ as a valuable source of protein[J].Food/Nahrung, 1982, 26 (5) :451-456.
[2] 顾尤.从玉米胚芽中综合提取油和蛋白粉工艺研究[J].中国食品添加剂, 2009, 9:146-149. [3] 唐文婷, 蒲传奋.双酶法提取玉米胚芽油工艺研究[J].粮油食品科技, 2009, 1:10-12. [4] 钱志娟, 王璋, 许时婴, 等.玉米胚芽水酶法提油及蛋白质的回收[J].无锡轻工大学学报, 2004, 23 (5) :58-23. [5] 王素梅, 王璋.水酶法提取玉米胚油工艺[J].无锡轻工大学学报, 2002, 21 (5) :482-486. [6] Zayas J F, lin C S.Water rentention of types of hexanedefatted corn germ proteins and soybean protein flour[J].Cereal chemistry, 1989, 66:51-55.
[7] Hung S C, Zayas J F.Protein solubility, water retention, and fat binding of corn germ protein flour compared with milk proteins[J].Journal of food science, 1992, 57 (2) :372-376.
[8] Zayas J F, Lin C S.Emulsifying properties of corn germ proteins[J].Cereal chemistry, 1989, 66 (4) :263-267.
[9] 陆晓滨.玉米胚芽蛋白质的酶解及在酱油生产中的应用[J].中国调味品, 2003, 2:15-18. [10] Tsen C C, Mojibian C N, Inglett G E.Defatted corn-germ flour as a nutrient fortifier for bread[J].Cereal Chemistry, 1974, 51:262.
[11] 高景华.脱脂玉米胚芽蛋白饮料的研制[J].食品工业科技, 2000, 21 (5) :53-54. [12] Peri C, Barbieri R, Casiraghi E M.Physical, chemical and nutritional quality of extruded corn germ flour and milk protein blends[J].International Journal of Food Science&Technology, 1983, 18 (1) :43-52.
[13] 马晓军.玉米胚芽汁生产实验[J].食品工业科技, 1998, (5) :43-44. [14] 刘丽娜, 王文亮, 张奇志, 等.玉米胚芽蛋白提取工艺研究[J].中国食物与营养, 2011, 17 (12) :60-63. [15] 陈晔, 谭天伟.酶法水解棉籽蛋白的制备与应用[J].北京化工大学学报:自然科学版, 2003, 30 (5) :25-27. [16] 胡睿娟, 郝利平.分步酶解法提取扁桃仁油及水解蛋白研究[J].山西农业科学, 2012, 40 (2) :156-160, 163. [17] 朱志方, 章绍兵, 陆启玉.水酶法同时提取小麦胚芽油及蛋白质的研究[C]//Proceedings of 2010 First International Conference on Cellular, Molecular Biology, Biophysics and Bioengineering (Volume 6) .2010. [18] 金英姿, 庞彩霞.碱性蛋白酶水解玉米蛋白工艺条件的研究[J].食品研究与开发, 2006, 27 (3) :44-45;79. [19] 皮钰珍, 刘彦妮, 岳喜庆, 等.酶法提取玉米胚芽蛋白的工艺优化[J].食品科技, 2011, 36 (7) :157-159.
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