Study on inhibition effects of propolis extract from Volvariella volvacea and contaminative microorganisms
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摘要: 以云南蒙自的粗蜂胶为材料,采用75%乙醇、95%乙醇、75%乙酸乙酯、95%乙酸乙酯浸提,测定蜂胶提取液的黄酮含量,并进行草菇及分离获得的污染菌的抑制作用研究。结果表明,95%乙酸乙酯提取效果较佳,其可溶物含量为60.3%,黄酮含量为6.23mg/mL;分离获得的草菇污染菌初步鉴定为革兰氏阳性细菌和青霉属菌。蜂胶提取液对草菇及污染菌均有一定的抑制作用,抑制强弱为:细菌>草菇>霉菌。相关性分析表明,草菇、霉菌和细菌的抑制率与黄酮含量的相关系数均大于0.9,有较强的相关性。Abstract: Using crude propolis of Mengzi Yunnan as raw materials, different propolis extraction were acquired in 75% ethanol, 95% ethanol, 75% ethyl acetate and 95% ethyl acetate at room temperature.The flavonoids contents of propolis extract were determinated, and the inhibition of propolis extract on the Volvariella volvacea and contaminative microorganisms which isolated from contaminative V.volvacea strains was discussed.The results showed that the most soluble content was 60.3% and most flavonoids content was 6.23mg/mL, under the conditions of 95% ethyl acetate extraction.After preliminary identification, contaminative microorganisms were gram-positive bacteria and Penicillium.Propolis extract had inhibitory effect on both P.eryngii and contaminative microorganisms, the inhibition degree was bacteria>V.volvacea>Penicillium.Correlation analysis indicated that all of the correlation coefficients on the inhibitory rate of bacteria, V.volvacea and Penicillium with the flavonoids content were above 0.9, which showed a good correlation.
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Keywords:
- propolis extract /
- Volvariella volvacea /
- contaminative microorganisms /
- ethanol /
- ethyl acetate /
- flavonoids
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