Study on the deproteinization method for Opuntia dillenii Haw. crude polysaccharides
-
摘要: 采用三氯乙酸(TCA)法、Sevag法、蛋白质等电点法对仙人掌粗多糖进行脱蛋白研究,以蛋白脱除率、多糖损失率和抗氧化性损失率为指标。结果表明:三氯乙酸法脱蛋白效果最好,浓度为3%时,蛋白脱除率为80.28%,多糖损失率为5.98%,抗氧化性损失率为34.56%。Abstract: Trichloroacetic acid method, Sevag method and the protein isoelectric point method were used to remove protein from Opuntia dillenii Haw.crude polysaccharides.And deproteinization effects of this three methods were compared by the loss of protein, the loss of polysaccharides and the loss of inoxidizability.Results showed that with concentration of trichloroacetic acid 3%, deproteinization was most effective.In this condition, the loss of protein was 80.28%, the loss of polysaccharides was 5.98% and the loss of inoxidizability was 34.56%.
-
Keywords:
- Opuntia dillenii Haw. /
- polysaccharides /
- deproteinization /
- inoxidizability
-
[1] Bohm H.“Opuntia dillenii”-An interesting and promising cactaceae taxon[J].J PACD, 2008 (10) :148-170.
[2] Yang N, Zhao M M, Zhu B H, et al.Anti-diabetic effects of polysaccharides from Opuntia megacantha cladode in normal and streptozotocin-induced diabetic rats[J].Innovative Food Science&Emerging Technologies, 2008 (9) :570-574.
[3] Zhao L Y, Huang W, Yuan Q X, et al.Hypolipidemic effects and mechanisms of the main component of Opuntia dillenii Haw.polysaccharides in high-fat emulsion-induced hyperlipidemic rats[J].Food Chemistry, 2012, 134:964-971.
[4] 梁蓓蓓, 刘华钢, 曹俊涛.仙人掌果多糖对S180荷瘤小鼠抑瘤作用的实验研究[J].癌症, 2008, 27 (6) :580-584. [5] Huang X J, Li Q, Guo L J, et al.Protection of cactus polysaccharide against H2O2-induced damage in the rat cerebral cortex and hippocampus differences in time of administration[J].Neural Regen Res, 2008, 3 (1) :14-18.
[6] Schepetkin I A, Xie G, Kirpotina L N, et al.Macrophage immunomodulatory activity of polysaccharides isolated from Opuntia polyacantha[J].International Immunopharmacology, 2008, 8 (10) :1455-1466.
[7] 喻宁华, 曾富华, 饶力群.仙人掌多糖对小鼠急性肝损伤的保护作用[J].中国生化药物杂志, 2009, 30 (4) :255-258. [8] 袁清霞, 赵龙岩, 李子娇, 等.酸法提取仙人掌多糖工艺[J].食品研究与开发, 2012, 33 (3) :42-45. [9] 赵龙岩, 袁清霞, 李子娇, 等.仙人掌多糖的双酶法提取及含量测定方法优化[J].中国医药工业杂志, 2011, 42 (2) :96-100. [10] 梁艳, 应苗苗, 吕英华, 等.微波辅助提取仙人掌多糖的工艺研究[J].农业工程学报, 2006, 22 (7) :159-162. [11] Zhao L Y, Lan Q J, Huang Z C, et al.Antidiabetic effect of a newly identified component of Opuntia dillenii polysaccharides[J].Phytomedicine, 2011 (18) :661-668.
[12] 钱松祥, 石森林, 潘水珍.苯酚-硫酸法测定固元胶囊中多糖[J].中草药, 2006, 37 (8) :1180-1181. [13] 王文平, 郭祀远, 李琳, 等.考马斯亮蓝法测定野木瓜多糖中蛋白质的含量[J].食品研究与开发, 2008, 29 (l) :115-117. [14] 孟宪军, 刘晓晶, 孙希云, 等.蓝莓多糖的抗氧化性与抑菌作用[J].食品科学, 2010, 31 (17) :110-114. [15] 黄生权.赤灵芝多糖的提取分离、结构分析与生物活性研究[D].广州:华南理工大学, 2010. [16] 董英, 张艳芳, 孙艳辉.水飞蓟粗多糖脱蛋白方法的比较[J].食品科学, 2007, 28 (12) :82-84. [17] 李强, 唐微, 郑伟, 等.杜仲粗多糖脱蛋白方法的对比研究[J].食品工业科技, 2011, 32 (3) :315-317.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
