Study on the production of Enteromorpha prolifera bio-organic fertilizer and its effect on the quality of Cherry Radish
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摘要: 从腐烂的浒苔中筛选出2株细菌和1株真菌,经过基因测序鉴定为:蜡状芽孢杆菌、枯草芽孢杆菌和米根霉。将这3株菌与米曲霉复配成EM-L菌剂,并将该菌剂应用于浒苔（Enteromorpha prolifera）发酵。结果表明,该菌剂发酵浒苔具有升温迅速、温度高、周期短、组织降解彻底、无明显臭味的特点。发酵后的物料符合国家有机肥相关标准,可以作为肥料原料使用。将发酵物料和有机辅料通过配比研制的浒苔生物有机肥成分丰富,除含有作物所需的氮、磷、钾等大量元素外,还具有浒苔活性多糖和藻朊酸等小分子有机物及钙、镁、碘、锌等微量元素。以樱桃萝卜（Cherry Radish）为研究对象,施用4t/hm2的浒苔生物有机肥相对于对照有机肥能明显增加樱桃萝卜的重量、直径、还原糖含量和叶绿素含量,具有提高樱桃萝卜的产量和品质的作用。另外,还可以减少无机肥的施用量,改善土壤微生物环境,提高土壤有机成分含量,充分利用废弃资源,达到减少对环境污染的目的。Abstract: Two strains of bacteria and one strain of fungus were isolated from decayed Enteromorpha proliferat.Based on the gene sequence the microorganisms were Bacillus cereus, Bacillus subtilis, and Saccharomyces cerevisiae, respectively.The complex microbial inoculants named as EM-D (including Bacillus cereus, Bacillus subtilis, Saccharomyces cerevisiae and one strain of Aspergillus oryzae) was applied on the experiment of Enteromorpha prolifera fermentation.The results showed the fermentation process possesses characteristics of warming rapidly, high temperature, short period, completely degradation of the tissue and indistinctive odor.The fermentation production meets the relevant standards of the national organic fertilizer and could be used as fertilizer directly.Green algae bio-organic fertilizer which was produced by the fermentation production and organic materials was rich in N, P, K.The fertilizer was also composed of active polysaccharides, amino acid and the microelement of I, Mg, Mn, Ti.Compared to the control group, the fertilizer amount of 4t/hm2could significantly increase the weight, diameter, reduce sugar content and chlorophyll content of Cherry Radish.At the same time, the application of green algae biological fertilizer could reduce of chemical fertilizer usage, improve soil microbial environment, and increase of soil organic component content.
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Keywords:
- Enteromorpha prolifera /
- fermentation /
- Cherry Radish /
- quality
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