Optimization of the extraction technology of the triterpenoid from inonotus hispidus by response surface
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摘要: 为提高粗毛纤孔菌三萜物质的得率,本研究首先通过单因素实验,确定影响提取的四个主要因子:溶剂体积分数、提取温度、料液比、提取时间的最佳条件。然后采用响应面分析法,研究各因子及它们的相互作用对三萜得率的影响。发现各因子对其三萜得率作用的先后顺序为:溶剂体积分数>提取温度>料液比>提取时间,且提取温度和提取时间的交互作用对三萜的得率存在显著性作用。并模拟二次回归方程,预测三萜最优的提取参数为:乙醇体积分数73%、提取温度67℃、料液比(g/mL)1∶53.0、提取时间5.25h时,三萜的得率达4.59%。Abstract: In order to improve the extraction yield of triterpenoid from inonotus hispidus, the extraction technology of 4 main factors (ethanol concentration, temperature, solid-to-liquid ratio and time) were optimized based on single factor.Then the effects of the 4 factors and their interactions on triterpenoid extraction yield were studied by response surface.The most important factor influencing the extraction yield was ethanol concentration, followed by temperature, solid-to-liquid and extraction time.The interaction of extraction temperature and time had significant role in extraction yield of triterpenoid from inonotus hispidus.And quadratic regression equation was simulated, and the best extraction parameters were:ethanol concentration 73%, extraction temperature 67℃, solid- to- liquid ratio 1∶53.0, extraction time5.25h, and the extraction yield reached 4.59%.
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Keywords:
- inonotus hispidus /
- triterpenoids /
- extraction /
- response surface
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