Optimization of preparation process for fish scale collagen peptide calcium chelates
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摘要: 采用响应面法对罗非鱼鳞胶原蛋白肽钙螯合物的制备工艺进行优化,建立了肽钙螯合率与时间、肽与氯化钙质量比、温度、pH的数学模型。最佳制备参数为:时间39min、肽与氯化钙质量比3.3∶1、温度61℃和pH7.2,此条件下肽钙螯合率的验证值为65.01%,与预测值(65.05%)无显著性差异。红外光谱分析表明罗非鱼鳞胶原蛋白肽与钙形成了螯合物。为新型钙制剂的开发及鱼鳞的高值化利用提供了理论依据。Abstract: The preparation process for tilapia scale collagen peptide calcium chelate was optimized using response surface methodology, and a mathematical model was established to describe peptide calcium chelate ratio as a function of time, mass ratio of peptide to calcium chloride, temperature and pH. The optimal preparation parameters obtained were as follows:time 39min, mass ratio of peptide to calcium chloride 3.3∶1, temperature 61℃ and pH7.2. Under these conditions, the experimental value was 65.01%, which was no significant difference with the predicted value ( 65. 05 %) . Infrared spectroscopy showed that tilapia scale collagen peptide calcium chelate was formed. These experimental results provided a theoretical basis for the development of new calcium preparations and the high-value utilization of fish scales.
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