Simultaneous determination of nine preservatives in fruits and vegetables by high performance liquid chromatography with programmed wavelength UV detection
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摘要: 建立了柑橘、黄瓜、白菜、西红柿四种果蔬中9种防腐剂（对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯、对羟基苯甲酸异丙酯、对羟基苯甲酸异丁酯、乙萘酚、乙氧基喹、4-苯基苯酚）的程序变波长-高效液相色谱同时测定方法。样品采用乙醚提取,液-液萃取净化,以甲醇-水为流动相进行梯度洗脱,经Agilent Eclipse XDB-C18色谱柱（150mm×4.6mm,3.5μm）分离,程序化变波长检测,外标法定量。结果表明,9种防腐剂在0.5100.0mg/L范围内线性关系良好（r≥0.9991）;在50、200、500μg/kg三个添加水平下平均回收率为70.7%119.5%,相对标准偏差（RSD）为0.9%9.9%。该方法简便快速、准确可靠,适用于果蔬中多种防腐剂残留的同时测定。Abstract: A method of simultaneous determination of nine preservatives, namely, paraben preservatives (methyl paraben, ethyl paraben, propyl paraben, butyl paraben, isopropyl paraben, isobutyl paraben, 2-naphthalenol, ethoxyquin and p- phenylphenol) in orange, cucumber, cabbage and tomato by high performance liquid chromatography with programmed wavelength UV detection was developed. The samples were extracted with diethyl ether, then cleaned up with liquid-liquid extraction, separated with a Agilent Eclipse XDB-C18 column (150mm×4.6mm, 3.5μm) using methol-water as mobile phase with a gradient elution program, determined with variable wavelength detector, and quantified with external standard curve. The nine preservatives had good linear relationships in the range of 0.5100.0mg/L, the correlation coefficients were higher than 0.9991. Average recoveries of the nine preservatives at spiking levels of 50, 200, 500μg/kg were 70.7% 119.5% and relative standard deviations were 0.9%9.9%. The method developed is simple, rapid and accurate, and can be used for the determination of trace residues of nine preservatives in fruits and vegetables.
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