Study on antioxidative activity and stability on the refined and high purity of anthocyanins of Lonicera edulis
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摘要: 本实验对提取的精制及高纯度的蓝靛果花色苷进行了抗氧化性及稳定性研究,旨在为其开发利用提供理论依据。抗氧化方面,对DPPH·的清除率、羟自由基（·OH）的清除率、总还原力进行测定及对脂质过氧化抑制进行评价,结果表明这两种花色苷对DPPH·的清除和脂质过氧化抑制方面效果较好都强于VC,总还原力及·OH的清除方面虽弱于VC但都有一定作用,且高纯度花色苷较精制花色苷的效果更好;稳定性方面,研究了光、温度、常用的几种食品添加剂、各种金属离子对这两种花色苷稳定性的影响及它们的耐氧化耐还原性等,结果表明这两种花色苷在温度≤60℃及暗处的保存效果更好;两种花色苷对氧化剂和还原剂都较敏感,有一定的耐氧化性,耐还原性不强;多数金属离子对这两种花色苷影响不大或具有增色作用,少数金属离子如Cu2+、Fe2+、Fe3+对这两种花色苷有破坏作用;NaCl、柠檬酸对这两种花色苷有增色作用,VC、山梨酸钾、苯甲酸钠有减色作用,蔗糖影响不大;且高纯度花色苷较精制花色苷稳定性要差。综上所述,高纯度花色苷抗氧化效果强于精制花色苷,稳定性不如精制花色苷。Abstract: The experiments studied antioxidation and stability of the refined and high purity anthocyanins from Lonicera edulis, in order to provide theoretical basis for its development and utilization.On the aspect of antioxidation, they were evaluated by scavenging capacity of·OH and DPPH·, the total reducing power, lipid peroxidation inhibition.The results showed that these two anthocyanins were stronger than that of VC on scavenging of DPPH· and lipid peroxidation inhibition, and the total reduction power and OH·scavenging capacity were weaker than that of VC but had a certain effect, and the high purity anthocyanin was better than the refined anthocyanin;on the aspect of stability, we studied on them by the effect of sunlight, several kinds of food additives, all kinds of metal ions, their oxidation and reducibility resistance, the results showed that the two anthocyanins have better preservation when the temperature was less than 60℃ and in dark;two anthocyanins were sensitive to both oxidizing and reducing agents, and had a certain resistance to oxidation but not strong reduction of resistance;two anthocyanins were relatively stable for the vast majority of metal ions, few metal ions such as Cu2 +, Fe2 +, Fe3 +had damage to them;NaCl, citric acid had the hyperchromic effect to these two anthocyanins, VC, potassium sorbate, sodium benzoate had hypochromic effect, sucrose had little effect;and high purity anthocyanin was worse than the refined anthocyanin on stability.To sum up, high purity anthocyanin had better antioxidant effect compared to the refined anthocyanin, but had worse stability than refined anthocyanin.
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Keywords:
- Lonicera edulis /
- anthocyanin /
- antioxidant /
- stability
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