Effect of quercetin on learning and memory of mice fed high fat diet
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摘要: 探讨槲皮素对高脂日粮小鼠学习记忆的影响及可能机制。4周龄C57B/L雌性小鼠45只,随机分为三组:对照组（基础日粮）,高脂组（约20%脂肪）,槲皮素组（高脂日粮+0.01%槲皮素）。第26周进行Morris水迷宫实验,实验结束后取小鼠大脑皮层和海马组织测定氧化应激指标,荧光定量PCR检测海马组织中脑源性神经营养因子（BDNF）、核转录因子Nrf2及血红素单加氧酶（HO-1）的基因表达。结果显示,高脂组小鼠学习记忆能力显著下降,脑组织出现明显的氧化应激,Nrf2、HO-1和BDNF基因表达显著下调;槲皮素干预后,大脑氧化应激程度减轻,Nrf2、HO-1和BDNF基因表达上调,学习记忆能力得到显著改善。槲皮素改善高脂日粮小鼠学习记忆,可能与上调Nrf2/HO-1通路及BDNF的基因表达有关。Abstract: To explore the effect of quercetin on learning and memory in C57B /L mice fed high-fat diet and potential mechanism.45 female C57B /L mice were randomly divided into three groups: control group (normal diet) , high-fat diet group (about 20% fat) , quercetin group (high-fat diet supplemented with 0.01% quercetin) .Morris water maze experiment was conducted at the 26thweek.After that, mice were sacrificed.Then oxidative stress indexes in cortex and hippocampus were tested and the expression of NF-E2-related factor 2 (Nrf2) , Heme oxygenase-1 (HO-1) and Brain derived neurotrophic factor (BDNF) in hippocampus were evaluated by RT-PCR.Compared to the control group, there was a significant deficit in learning and memory in HFD group (p < 0.05) .The cortex and hippocampus of mice fed high-fat diet were exposed to significant oxidative damage (p < 0.05) .Furthermore, the expression of Nrf2, HO-1 and BDNF in hippocampus tissue decreased significantly (p < 0.05) in HFD group.After treated with quercetin, oxidative damage of cortex and hippocampus was alleviated.Additionally, the expression of Nrf2, HO-1 and BDNF were up-regulated and learning and memory was improved significantly (p < 0.05) .Quercetin could improve high-fat diet induced memory and learning impairment, which might be attributed to upregulate the expression of Nrf2, HO-1 and BDNF.
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Keywords:
- high-fat diet /
- oxidative stress /
- quercetin /
- NF-E2-related factor 2 (Nrf2) /
- learning and memory
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