Study on extraction and detection of folic acid in millet
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摘要: 研究小米叶酸的最佳提取条件与测定方法。以河北红谷小米为原料,针对光照条件、显色时间、检测范围、提取时间、吸附剂用量、洗脱液用量和加标回收分别进行叶酸提取的单因素实验,确定小米叶酸的最佳提取条件:用0.1mol/L磷酸二氢钾25mL浸泡2.5g小米,50℃恒温水浴8h,0.5g活性炭（苯胺处理）吸附10min,3%氨-70%乙醇洗脱5次,每次用量7mL。测定方法是用高锰酸钾氧化叶酸,间接荧光分析法测定。该法操作简单,稳定性好,灵敏度高,回收率为96.14%,RSD为1.68%,结果令人满意。Abstract: The optimum extraction condition and measurement method for analysis of folic acid in millet was studied.The red valley millet cultivar from Hebei was used as materials, and a single factor experiment for folic acid extraction and detection was respectively conducted based on the illumination condition, color rendering time, detection ranges, extraction time, usage of absorbent, the consumption amount of elution solution as well as the recovery rate of standard sample.Data showed that 25mL of 0.1mol/L KH2 PO4 was used to obtain the optimum extraction effect by soaking 2.5g millet at 50℃ for 8 hours, and adsorbing for 10 minutes with 0.5g active carbon adsorbent treated by aniline, and continuously eluting five times with 7mL of mixture containing 3% ammonia and 70% ethanol, and then indirect fluorescent method was used to determine the contents of folic acid oxidized by KMnO4, and the satisfactory result was obtained because of higher the recovery rate of 96.14% with a relative lower deviation of 1.68%.
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Keywords:
- millet /
- folic acid /
- extraction /
- measurement /
- indirect fluorescent method
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