Extraction of dietary fiber from Ganoderma atrum with response surface optimization
-
摘要: 以黑灵芝为原料,采用酶法和化学法联用,从黑灵芝中提取出可溶性膳食纤维和不可溶性膳食纤维,借助响应面设计分析,考察酶解温度、酶解时间、料液比和碱提pH、碱提温度、碱提时间分别对黑灵芝可溶性膳食纤维(SDF)、不可溶性膳食纤维(IDF)得率的影响。结果表明,最佳提取工艺条件分别为:酶解温度98℃、酶解时间103min、料液比1∶32和碱提pH10、碱提温度48℃、碱提时间41min,在此条件下黑灵芝SDF得率为1.08%,IDF得率为88.68%。Abstract: Response surface methodology was applied to optimize the extraction conditions ofsoluble dietary fiber (SDF) and insoluble dietary fiber (IDF) from Ganoderma atrum.Effects of enzymolysis temperature, reaction time and material-liquid ratio on the yield of SDF and alkaline extraction pH, temperature and time on the yield of IDF were investigated.Results exhibited that the optimum extraction conditions were as follows:enzymolysis temperature and reaction time was 98℃ and 103min, solid-liquid ratio was 1∶32, alkaline extraction pH, temperature and time was 10, 48℃, 41min.The yield of SDF and IDF was 1.08% and 88.68%, respectively, under optimized conditions.
-
[1] 黄凯丰, 杜明凤.膳食纤维研究进展[J].河北农业科学, 2009, 13 (5) :53-55. [2] 王淑红.膳食纤维对控制血糖的作用[J].中华中西医学杂志, 2006, 4 (11) :117. [3] 袁尔东, 郑建仙.不同品种膳食纤维降血脂功能的比较[J].中国粮油学报, 2002, 17 (3) :38-41. [4] 冯丽君, 吴巧凤.膳食纤维的保健作用与分析方法研究进展[J].浙江预防医学, 2008, 20 (7) :59-60. [5] Phillips G O, Cui S W.An introduction:evolution and finalisation of the regulatory definition of dietary fibre[J].Food Hydrocolloids, 2011, 25 (2) :139-143.
[6] 曹荣安, 贾建, 李良玉, 等.善食纤维的生理功能特性曼其在食品工业中的应用[J].肉类研究, 2010 (2) :76-78. [7] Elleuch M, Bedigian D, Roiseux O, et al.Dietary fibre and fibre-rich by-products of food processing:Characterisation, technological functionality and commercial applications:A review[J].Food Chemistry, 2011, 124 (2) :411-421.
[8] 丁晨阳, 韩舜愈, 盛文军, 等.响应曲面法优化苹果渣可溶性膳食纤维提取工艺[J].食品工业科技, 2012, 33 (7) :266-270. [9] 李昊虬, 王国泽, 孙晓宇.响应面法优化河套蜜瓜皮水溶性膳食纤维提取工艺的研究[J].食品工业科技, 2012, 33 (7) :254-256. [10] 宋燕, 罗松明, 向建军, 等.响应面法优化花椒籽不可溶性膳食纤维提取工艺研究[J].中国油脂, 2011, 36 (7) :62-67. [11] He C Y, Li W D, Guo S X, et al.Effect of polysaccharides from Ganoderma lucidum on streptozotocin-induced diabetic nephropathy in mice[J].Journal of Asian Natural Products Research, 2006, 8 (8) :705-711.
[12] 张汇, 鄢嫣, 聂少平, 等.黑灵芝不同部位多糖成分分析及抗氧化活性[J].食品科学, 2011, 32 (1) :56-61. [13] 李建军, 雷林生, 余传林, 等.灵芝多糖抗肿瘤作用的免疫学相关性研究[J].中药材, 2007, 30 (1) :71-73. [14] 李文娟, 聂少平, 余强, 等.黑灵芝多糖对免疫抑制小鼠的免疫调节作用[J].食品科学, 2009, 30 (19) :297-299. [15] 吴笑臣, 王科军, 钟金莲, 等.响应面法优化脐橙渣中水溶性膳食纤维提取工艺[J].食品科学, 2012, 33 (4) :109-114. [16] 李加兴, 梁先长, 黄诚, 等.响应面法优化火棘水不溶性膳食纤维提取工艺[J].食品科学, 2011, 32 (14) :118-123.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
